Please use this identifier to cite or link to this item:
http://repo.knmu.edu.ua/handle/123456789/29912
Title: | Experimental Evaluation of Food-Grade Semi-Refined Carrageenan Toxicity |
Authors: | Pogozhykh, Denys Posokhov, Yevgen Myasoedov, Valeriy Gubina-Vakulyck, Galina Chumachenko, Tetyana Knigavko, Oleksandr Polikarpova, Hanna Kalashnyk-Vakulenko, Yuliia Sharashydze, Ketino Nakonechna, Oksana Prokopyuk, Volodymyr Onishchenko, Anatolii Tkachenko, Anton |
Keywords: | carrageenan E407a processed Eucheuma seaweed toxicity fluorescent probe leukocytes inflammation intestine animal model |
Issue Date: | 16-Oct-2021 |
Citation: | Experimental Evaluation of Food-Grade Semi-Refined Carrageenan Toxicity / D. Pogozhykh, Y. Posokhov, V. Myasoedov, G. Gubina-Vakulyck, T. Chumachenko, O. Knigavko, H. Polikarpova, Y. Kalashnyk-Vakulenko, K. Sharashydze, O. Nakonechna, V. Prokopyuk, A. Onishchenko, A. Tkachenko // International Journal of Molecular Sciences. – 2021. – Vol. 22. – P. 11178. |
Abstract: | Abstract: The safety of food additives E407 and E407a has raised concerns in the scientific community. Thus, this study aims to assess the local and systemic toxic effects of the common food additive E407a in rats orally exposed to it for two weeks. Complex evaluations of the effects of semi-refined carrageenan (E407a) on rats upon oral exposure were performed. Local effects of E407a on the intestine were analyzed using routine histological stains and CD68 immunostaining. Furthermore, circulating levels of inflammatory markers were assessed. A fluorescent probe O1O (2- (20-OH-phenyl)-5-phenyl- 1,3-oxazole) was used for evaluating the state of leukocyte cell membranes. Cell death modes of leukocytes were analyzed by flow cytometry using Annexin V and 7-aminoactinomycin D staining. Oral administration of the common food additive E407a was found to be associated with altered small and large intestinal morphology, infiltration of the lamina propria in the small intestine with macrophages (CD68+ cells), high systemic levels of inflammation markers, and changes in the lipid order of the phospholipid bilayer in the cell membranes of leukocytes, alongside the activation of their apoptosis. Our findings suggest that oral exposure to E407a through rats results in the development of intestinal inflammation. |
URI: | https://repo.knmu.edu.ua/handle/123456789/29912 |
Appears in Collections: | Наукові праці. Науково-дослідний інститут експериментальної та клінічної медицини ХНМУ |
Files in This Item:
File | Description | Size | Format | |
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IJMS_MDPI_2021.pdf | 3,99 MB | Adobe PDF | View/Open |
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