Browsing by Author "Kapustnyk, Nataliia"
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Item Angiotensin-converting enzyme 2 in mucous membrane of small and large intestines in patients in post-COVID-19 period(2022-10-21) Myroshnychenko, Mykhailo; Kapustnyk, Nataliia; Arseniev, Oleksandr; Molodan, DmytroItem Chronic inflammatory processes of the maternal genitourinary system, its role in cardiovascular diseases development in their children: Ukrainian and European experience of prevention as the key to the health of future generations(2023) Olianych, Serhii; Myroshnychenko, Mykhailo; Pasiyeshvili, Nana; Kapustnyk, Nataliia; Bibichenko, VictoriaCardiovascular diseases (CVDs) remain the leading cause of morbidity in the world. The burden of CVDs continues to increase over the past decade in almost all countries of the world. The prevalence of CVDs in the world has almost doubled from 271 million i 1990 to 523 million in 2019. The number of deaths from CVDs over the same period has increased by twofold from 12.1 million to 18.6 million. The indicators of years of life with disability and lost years also doubled in total from 17.7 million in 1990 to 34.4 million in 2019. Data from Ukrainian and European statistics testify to the need for timely active measures aimed at preventing the development of CVDs. Modern strategies for the prevention of CVDs are primarily aimed at correcting the known risk factors: quitting smoking, regular physical exercise, normalizing body mass index, glucose, normalizing blood pressure, etc. What is not taken into account in this strategy? The very first periods of a human life, which significantly program health, duration and quality of life, remain beyond attention. In the structure of genital and extragenital pathology of women of reproductive age, significant parts are inflammatory diseases of the genitourinary system, which are characterized by predominantly chronic course. Simultaneous development of inflammatory diseases of the urinary and reproductive systems is explained by the facts that these systems organs develop from one primary germ layer (mesoderm), they have a close anatomical-physiological connection, a common blood supply and lymphatic system, and are also target organs of sex hormones activity. Babies born prematurely are at a greater risk of developing heart failure later in life. The incidence of heart failure in individuals born extremely prematurely (less than 28 weeks gestation) was 17.0 times higher compared to those born at term (37 weeks or more), after taking into account other factors. Similarly, those born very prematurely (28 to 31 weeks gestation) had an incidence of heart failure 3.58 times higher compared to term births. The analysis of the research results of Ukrainian and European scientists has shown that chronic inflammatory processes of the genitourinary system of the mother are a significant damaging factor that can change the morphological and functional state of the fetal and newborn cardiovascular system and cause the development of CVDs at various stages of ontogenesis. Thus, chronic inflammatory processes of the mother's genitourinary system are one of the factors that can cause the development of CVDs in children at different stages of ontogenesis. The classic concept of CVDs prevention should be supplemented taking into account the programming effect of harmful factors that act on the preconception and prenatal stages and reliably increase the risk of CDVs development. Among the reproduktive age, it is extremely necessary to carry out measures aimed at prevention, timely diagnostics and treatment of infectious and inflammatory diseases of the genitourinary system.Item Compensatory and regenerative potential in kidneys of newborns from mothers with complicated pregnancy by preeclampsia and iron deficiency anemia(2022-09-03) Myroshnychenko, Mykhailo; Kapustnyk, Nataliia; Shapkin, Anton; Moiseienko, Tetiana; Torianyk, Inna; Ivannik, Victoria; Chastii, Tetiana; Mozhaiev, IvanItem Expression features of human papilloma virus type 16 and anti- Epstein-Barr virus in pleomorphic adenoma, surrounding and intact salivary gland(2021-08) Brodetskyi, Igor; Malanchuk, Vladislav; Dyadyk, Olena; Myroshnychenko, Mykhailo; Kulbashna, Kulbashna; Astapenko, Olena; Feleshtynska, Oksana; Kapustnyk, Nataliia; Kalashnyk-Vakulenko, YuliiaThe aim is to reveal the expression features of MCA to human papilloma virus type 16 and anti-Epstein-Barr virus in the pleomorphic adenoma, surrounding and intact salivary gland. The severity of the expression of these markers in the salivary gland is determined by the histological variant of the tumor (severely expressed in the epithelial variant, moderately expressed in the mixed variant, and minimally expressed in the mesenchymal variant). The immunohistochemical study has shown that the Epstein-Barr virus and, especially, human papilloma virus type 16 can act as exogenous trigger factors involved in the development of pleomorphic adenoma of the salivary glands. The revealed immunohistochemical features of MCA expression to human papilloma virus type 16 and anti- Epstein-Barr virus in the salivary gland surrounding the pleomorphic adenoma and in the intact tissue of the salivary gland make it possible to recommend the extracapsular dissection of the tumor with resection of the adjacent intact tissue of the salivary gland at a distance of 10 mm in patients with pleomorphic adenoma.Item Gender portrait of the COVID-19 infection: analysis of the problem(2021-04) Myroshnychenko, Mykhailo; Kuye, Alesegum; Kapustnyk, NataliiaItem Malignant tumors of the appendix: clinical and morphological analysis of cases from the practice(2021-05) Myroshnychenko, Mykhailo; Dyadyk, Olena; Kapustnyk, Nataliia; Fedulenkova, Yuliia; Borzenkova, Iryna; Astapieva, Olha; Selivanova, Larisa; Zakharenko, Valentyna; Lytvynenko, Olena; Molodan, Dmytro; Paskevych, Olga; Akritova, Kristina; Melnik, Bohdan; Bobrova, VladyslavaThe authors have analyzed medical histories of two patients, treated in health care facilities of Kharkiv region from 2008 to 2020. These patients underwent urgent appendectomy, given the existing clinic of acute appendicitis. Morphological examination of the surgical material allowed us to diagnose adenocarcinoma in one case, and neuroendocrine tumor in combination with endometriosis in the other case. Morphological examination of the surgical material in the first case revealed a moderately differentiated adenocarcinoma and diffuse neutrophilic infiltration in all layers of the appendix, and in the second case – a well-differentiated neuroendocrine tumor (G3), combined with the signs of phlegmonous-ulcerative appendicitis and loci of endometriosis. In both cases, there were no specific for the oncological process anamnestic and clinical-instrumental data, and these tumors were manifested by the clinic of acute appendicitis. Only morphological examination of the surgical material allowed identifying the pathological process. Clinical and morphological analysis of cases from the practice of malignant tumors of the appendix (neuroendocrine tumor and adenocarcinoma) will be useful and interesting for the medical community and should stimulate cancer vigilance in physicians.Item Morphological assessment of the lungs in post-COVID-19 syndrome: analysis of autopsy material(2023) Myroshnychenko, Mykhailo; Pasiyeshvili, Nana; Kapustnyk, Nataliia; Markovskiy, Volodymyr; Kalashnyk-Vakulenko, Yuliia; Zaytseva, Olga; Bondarenko, Maryna; Morozov, Oleksandr; Molodan, DmytroThe aim was to reveal the morphological features of the lungs in post-COVID-19 syndrome. Materials and methods: The material of the study was autopsy material – fragments of the lung tissue from 96 deceased (59 men and 37 women). During the lifetime, all patients had in anamnesis COVID-19 of varying severity, and after the treatment of this infection, they had various manifestations of respiratory failure until death. The average duration of the post-COVID-19 period was 148.6±9.5 days. Based on the severity of COVID-19 in anamnesis, all cases were divided into three groups. Group 1 included 39 cases with mild COVID-19 in anamnesis. Group 2 included 24 cases with moderate severity of COVID-19 in an¬amnesis. Group 3 included 33 cases with severe COVID-19 in anamnesis. Histological, histochemical, morphometric and statistical research methods were used. Results: Morphological features of the lungs in post-COVID-19 syndrome were the presence of pneumosclerosis; focal-diffuse immune cells infiltration; emphysematous and atelectatic changes; degenerative-desquamatic changes in the alveolar epithelium; metaplastic changes of connective tissue; dystrophic calcification; dystrophic, metaplastic and dysplastic changes in the epithelial layer of bronchial tree; hemodynamic disorders. Pneumosclerosis, focal-diffuse immune cells infiltration, alterative changes in the alveolar epithelium, emphysematous and atelectatic changes, hemodynamic disorders increased with an increase the severity of COVID-19. Metaplastic changes of connective tissue, dystrophic calcification, dystrophic, metaplastic and dysplastic changes in epithelial layer of bronchial tree did not depend on the severity of the infection. Conclusions: The changes identified by the authors help to explain pulmonary manifestations of post-COVID-19 syndrome. They should be the basis for the oncological alertness formation among doctors, the development of rehabilitation and treatment measures for such category of patients.Item Morphological features of local immune reactions in placentas in women who had COVID-19 disease in early gestation(2022) Myroshnychenko, Mykhailo; Gaponova, Ludmila; Kapustnyk, Nataliia; Gaponova, Elina; Borzenkova, Iryna; Pavlova, Olena; Myroshnychenko, SerhiiItem Morphological features of the lungs in post-COVID-19 period(2023) Myroshnychenko, Mykhailo; Sukhorukov, Ihor; Dyadyk, Olena; Pasiyeshvili, Nana; Kapustnyk, Nataliia; Ziablitsev, DenysItem Morphological justification of the salivary gland tissue resection boundaries during surgical treatment of patients with pleomorphic adenomas(2021-09) Brodetskyi, Igor; Malanchuk, Vladislav; Dyadyk, Olena; Kulbashna, Yaroslava; Astapenko, Olena; Kapustnyk, Nataliia; Myroshnychenko, MykhailoThe aim is to substantiate morphologically the resection boundaries of the salivary gland tissue in the surgical treatment of patients with pleomorphic adenoma of different histological variants. These changes were: violation of the ratio of the specific volumes of the parenchyma and stroma; atrophy of the terminal sections and ducts with cystic expansion of some ducts; thickening of the secretion and formation of calculi in the lumen of some ducts; atrophic and alterative changes in the epithelial lining the terminal sections and ducts; sclerosis and lipomatosis, areas with hyalinosis and dystrophic calcification in the stroma; hemodynamic disturbances in the stroma with a decrease in the number of vessels; pronounced focal or diffuse immune infiltration in the stroma in some areas with the lymphoid follicles formation.The comprehensive study has confirmed that removal of the tumor with the adjacent tissue of the salivary gland at a distance of 1.0 cm in patients with pleomorphic adenoma of various histological variants is the most justified from the morphological point of view.Item Rhinosinusitis in patients in post-COVID-19 period: etiology, clinical and morphological characteristics(2022-08-02) Myroshnychenko, Mykhailo; Kalashnyk-Vakulenko, Yuliia; Kapustnyk, Nataliia; Babycheva, Oleksandra; Yurevych, Nadiia; Bondarenko, Olha; Kalashnyk, Mykhailo; Torianyk, Inna; Shapkin, Anton; Lupyr, AndriiIntroduction. Rhinosinusitis is one of the otorhinolaryngological manifestations of the post-COVID-19 syndrome. The etiopathogenesis of such pathology is not well studied. The effectiveness of rhinosinusitis treatment in patients is determined by timely diagnosis and early implementation of etiopathogenetic therapy. Morphological examination of biopsy or surgical material is of great importance in the diagnosis of this pathology. The purpose is to identify the etiology, clinical and morphological features of rhinosinusitis in patients in post-COVID-19 period. Materials and methods. In the present study, it was carried out the analysis of 11 cases of rhinosinusitis, which developed after COVID-19 infection. The diagnosis of rhinosinusitis was established on the basis of anamnesis, clinical and laboratory examination, specialized instrumental examination (rhinoendoscopy, X-ray, magnetic resonance imaging, spiral and 3D computed tomography). All patients underwent endoscopic sanitation of the nasal cavity, expansion of the maxillary anastomosis, maxillary sinusotomy, sanitation of the maxillary sinuses and removal of pathologically altered tissues. Microbiological examination of the swab from the nasal cavity was carried out in all patients. Histological and morphometric research methods were used during the morphological study of surgical material. The nonparametric Mann-Whitney U test was used to compare the means in the groups. Results. The conducted comprehensive study made it possible to identify chronic atrophic rhinosinusitis at the stage of exacerbation caused by associations of bacteria and fungi in patients in post-COVID-19 period. Among bacteria, the authors most often noted Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia, Streptococcus pneumonia and Enterococcus faecalis. Among fungi, there were Aspergillus, Candida, Mucor and Coccidioides. Fungal infection was characterized by invasion into the mucous membrane of the nose and paranasal sinuses. In patients in post-COVID-19 period the invasive bacterial-fungal chronic atrophic rhinosinusitis at the stage of exacerbation was predominantly bilateral, characterized by the involvement of several or all paranasal sinuses in the process. Patients with such pathology complained of periodic fever, headaches and malaise; nasal congestion and constant difficulty in nasal breathing; yellowish-greenish-reddish discharge from the nasal cavity, sometimes with a fetid odor; discomfort and pain in the area of paranasal sinuses; immobility of the eyeball, hyposmia or anosmia; reduction or complete loss of vision. Frequent risk factors for the development of invasive bacterial-fungal chronic atrophic rhinosinusitis at the stage of exacerbation in patients in post-COVID-19 period were the information about moderate or severe course of this infection in anamnesis; comorbidities (predominantly diabetes mellitus, hypertensive disease and ischemic heart disease). Conclusions. The study conducted by the authors made it possible to identify the etiological, clinical and morphological features, as well as risk factors of rhinosinusitis in patients in post-COVID-19 period. This information will contribute to a better understanding of such pathology by the doctors and improve the diagnostic and treatment process.Item Scientific research in higher medical education institutions through the prism of war: ethical dimension(2023) Myroshnychenko, Mykhailo; Lazurenko, Viktoriya; Kapustnyk, Nataliia; Pasiyeshvili, Nana; Markovskyi, Volodymyr; Mishyna, MarynaItem Scientific research in higher medical education institutions through the prism of war: ethical dimension(2023) Myroshnychenko, Mykhailo; Lazurenko, Viktoriya; Kapustnyk, Nataliia; Pasiyeshvili, Nana; Markovskyi, Volodymyr; Mishyna, MarynaItem Special at-rich sequence-binding protein 2 and its role in healing of the experimental mandible bone tissue defect filling with a synthetic bone graft material and electrical stimulation impact(2024) Huseynov, Agil; Malanchuk, Vladislav; Myroshnychenko, Mykhailo; Kapustnyk, Nataliia; Sukharieva, Liliia; Selivanova, LarisaAim: The purpose of the study was to identify the role of SATB2 in healing of the experimental mandible bone tissue defect filling with a synthetic bone graft material and electrical stimulation impact. Materials and Methods: An experiment was carried out on 48 mature male rats of the WAG population, which were divided into 4 groups. Each group included 12 experimental animals. Group 1 included rats that were modeled with a perforated defect of the lower jaw body. Group 2 included animals that were modeled with a perforated defect similar to group 1. In animals, a microdevice for electrical action was implanted subcutaneously in the neck area on the side of the simulated bone defect. The negative electrode connected to the negative pole of the battery was in contact with the bone defect. The battery and electrode were insulated with plastic heat shrink material. Group 3 included rats that were modeled with a perforated defect similar to previous groups, the cavity of which was filled with synthetic bone graft “Biomin GT” (RAPID, Ukraine). Group 4 included animals that were modeled with a perforated defect similar to groups 1-3, the cavity of which was filled with synthetic bone graft “Biomin GT” (RAPID, Ukraine). The simulation of electrical stimulation was the same as in group 2. The material for the morphological study was a fragment of the body of the lower jaw from the zone of the perforated defect. Immunohistochemical study was performed using rabbit anti-human SATB2 monoclonal antibody. Results: In the regenerate filling the defect in the bone tissue of the lower jaw of rats, there was an increase in SATB2 expression under conditions of electrical stimulation; filling the defect with a synthetic bone graft material; simultaneous filling the defect with a synthetic bone graft material and electrical stimulation. The most pronounced expression of SATB2 was observed under conditions of simultaneous filling the defect with a synthetic bone graft material and electrical stimulation; minimally expressed – in conditions of filling the defect with a synthetic bone graft material; moderately expressed – under conditions of electrical stimulation. In the regenerate, in cases of all treatment methods, SATB2 was expressed by immune cells, fibroblastic differon cells, osteoblasts, and in case of electrical stimulation, also by adipocytes, vascular pericytes and endothelial cells, epidermis. Conclusions: The activation of SATB2 expression identified by the authors is one of the mechanisms for stimulating reparative osteogenesis under the conditions of electrical stimulation; filling the defect with a synthetic bone graft material; simultaneous filling the defect with a synthetic bone graft material and electrical stimulation. Мета: Мета дослідження – визначити роль SATB2 у загоєнні експериментального дефекту кісткової тканини нижньої щелепи, заповненому синтетичним кістковим трансплантатом та електростимуляційним впливом. Матеріали і методи: Експеримент проведено на 48 статевозрілих щурах-самцях популяції WAG, які були поділені на 4 групи. Кожна група включала 12 піддослідних тварин. До 1 групи увійшли щури, яким моделювався перфорований дефект тіла нижньої щелепи. До групи 2 увійшли тварини, яким моделювали перфораційний дефект, подібний до групи 1. Тваринам імплантували мікропристрій для електричної дії підшкірно в ділянку шиї з боку імітованого дефекту кістки. Негативний електрод, підключений до негативного полюса батареї, контактував з дефектом кістки. Акумулятор і електрод були ізольовані пластиковим термоусадковим матеріалом. 3 група включала щурів, яким моделювали перфорований дефект, подібний до попередніх груп, порожнину якого заповнювали синтетичним кістковим трансплантатом «Біомін ГТ» (РАПІД, Україна). До 4 групи увійшли тварини, яким моделювався перфорований дефект, подібний до груп 1-3, порожнину якого заповнювали синтетичним кістковим трансплантатом «Біомін ГТ» (РАПІД, Україна). Моделювання електростимуляції проводилося так само, як і в 2 групі. Матеріалом для морфологічного дослідження був фрагмент тіла нижньої щелепи із зони дірчастого дефекту. Імуногістохімічне дослідження проводили з використанням кролячих моноклональних антитіл проти людини SATB2. Результати: При заповненні регенератом дефекту кісткової тканини нижньої щелепи щурів спостерігалося підвищення експресії SATB2 в умовах електростимуляції; заповнення дефекту синтетичним кістково-трансплантаційним матеріалом; одночасне заповнення дефекту синтетичним кістковим трансплантатом і електростимуляція. Найбільш виражена експресія SATB2 спостерігалась за умов одночасного заповнення дефекту синтетичним кістковим трансплантатом та електростимуляції; мінімально виражені – в умовах заповнення дефекту синтетичним кістково-трансплантаційним матеріалом; помірно виражені – в умовах електростимуляції. У регенераті при всіх методах лікування SATB2 експресувався імунними клітинами, фібробластичними дифероновими клітинами, остеобластами, а при електричній стимуляції також адипоцитами, судинними перицитами та ендотеліальними клітинами епідермісу. Висновки: Виявлена авторами активація експресії SATB2 є одним із механізмів стимуляції репаративного остеогенезу в умовах електростимуляції; заповнення дефекту синтетичним кістково-трансплантаційним матеріалом; одночасне заповнення дефекту синтетичним кістковим трансплантатом і електростимуляція.Item Tumors and tumor-like lesions of the salivary glands: morphological characteristics of the surgical material(2021-04) Brodetskyi, Igor; Malanchuk, Vladislav; Sorokin, Bogdan; Myroshnychenko, Mykhailo; Beketova, Yuliya; Dyadyk, Olena; Kapustnyk, Nataliia; Krotevych, Mykhailo; Brodetskyi, SergeyThe aim is to give a morphological characteristic of tumors and tumor-like lesions of the salivary glands in the course of a comprehensive analysis of the surgical material. The connective tissue capsule separating the tumor tissue from the intact tissue of the salivary gland was clearly pronounced in adenolymphomas, and in pleomorphic adenomas it could be clearly expressed, intact, with tumor invasion or absent. In mesenchymal and mixed variants of pleomorphic adenomas, in comparison with the epithelial variant, there was a frequent tumor invasion of the capsule, thinning of the capsule or its absence. Morphological examination of the surgical material allows us to diagnose tumors (pleomorphic adenomas and adenolymphomas) and non-tumor lesions (cysts) of the salivary glands. This, of course, is of great therapeutic, diagnostic and prognostic value. Among the identified pathology of the salivary glands, pleomorphic adenomas cause certain difficulties in morphological diagnosis due to their structural diversity and heterogeneity, sometimes requiring morphometric, immunohistochemical or genetic research.