Кафедра біологічної хімії
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Browsing Кафедра біологічної хімії by Author "Böckelmann, Irina"
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Item Kombinierte Wirkung von Methyl-tert-butylether und Kälte auf innere Organe von Versuchstieren(Springer Medizin Verlag GmbH, 2019) Thielmann, Beatrice; Zavgorodnij, Igor; Kapustnik, Walerij; Bachynskyi, Ruslan; Batschinskaja, J.; Böckelmann, IrinaItem Toxizität von Methyl-tert-butylether auf innere Organe von Versuchstieren unter Kältebedingungen(2019) Zavgorodnij, Igor; Thielmann, Beatrice; Kapustnik, Walerij; Bachynskyi, Ruslan; Batschinskaja, J.; Böckelmann, IrinaBackground. The use of methyl tert-butyl ether (MTBE) is frequent. It can produce pollution of the environment and is a stress factor with potential health risks in workplaces. The simultaneous combined influence of a chemical and a physical stressor (MTBE and cold) can amplify the effects of MTBE. Workplaces with cold conditions are used in factories (e.g. chemical or construction industry). The aim of the work was the histological examination of the internal organs of rats under conditions of thermal comfort and in the cold. Material and methods. This toxicological study was carried out on 4 groups of 6 male rats. The 2 test groups were given 30 times MTBE in the stomach at a dose of 1/10 LD50 (500mg/kg of animal weight). The two control groups received distilled water. The groups were exposed to thermal comfort temperatures (25± 2 °C) or cold (4± 2 °C) for 4 h. Tissue samples were taken and histological examinations were made on day 30. Results. The pathological changes were significantly increased under exposure to cold conditions. Inflammatory reactions (e.g. glomerulonephritis), pulmonary emphysema and cytolytic signs of hepatocytes were found in the organs. The thyroid gland was hypothyroid. Reactive hyperplasia was detectable in the spleen. The adrenal border zones were barely definable and indicated a decreased functional activity of the nuclei of endocrine cells. Discussion. The histological studies showed a pronounced toxic potential of MTBE, which was significantly enhanced by cold conditions. Transferability to humans seems possible because MTBE in humans undergoes a similar degradation process as in rats. Therefore, special attention should be paid to protective measures at the workplace.Item Wirkung von Nitrobenzol auf innere Organe unter Kältebedingungen(2017-09) Zavgorodnij, Igor; Thielmann, Beatrice; Kapustnik, Walerij; Bachynskyi, Ruslan; Böckelmann, IrinaBackground and objectives. Certain workplaces show a combination of pollutant exposure and cold. There are indications that nitrobenzene is cytotoxic. The aim of this study was to investigate the toxic effects of nitrobenzene on organs, such as the liver, kidneys, adrenal, spleen and thyroid gland. Material and methods. These experiments were performed on 24 sexually mature male WAG rats. In each experimental group (2 × 6 animals), nitrobenzene was introduced 30 × (70 mg/kg of animal body weight) into the stomach 5 days per week for 6 weeks. The animals of the control group (2 × 6 animals) received an equivalent amount of distilled water. The animals were exposed to either thermal comfort (25 ± 2 °C) or cold conditions (4 ± 2 °C) for 4 h. After a 30-day recovery period, the data were re-elevated to investigate the question of reversibility. Results. Theeffectsofnitrobenzenein both experimental groups led to structural changes in the investigated organs; however, the structural changes were irreversible only in the experimental group under cold conditions. For example, there was evidence of acute hepatitis, activated immune responses in the spleen and thyroid gland and glomerulonephritis. Conclusion. The results of the animal experiments demonstrate an irreversible cytotoxic effect of nitrobenzene under cold conditions. Thus, the histological examination of organs can be considered as a target for the toxic effects of nitrobenzene.