Association between IL-2,IL-4 gene polymorphisms and pulmonary MDRTB

Abstract

Background.To study the association between IL-2,IL-4 gene polymorphisms and pulmonary MDRTB. Methods.The study comprised 170 individuals in Ukraine including 74 patients MDRTB(group1),66 patients without–MDRTB(group2) and 30 healthy donors(group3).Serum levels of cytokines IL-2 and IL-4(pg/L)were evaluated by ELISA.Two polymorphic variants were examined:T-330G promoter region of IL-2 and C-589T–IL-4genes. Results.In the 1 group the levels of IL-4 and IL-2 were 7.96±0.29 and 39.34±1.14,2nd–11.29±0.35 and 36.20±0.89 while in 3rd–29.99±1.27 and 21.60±0.80 respectively(p<0.05).In patients with MDRTB the heterozygous genotype (HeG)(79.73%(N=59) for IL-2 and 71.62%(N=53) for IL-4genes) was higher than: 10.81%(N=8) and 14.86%(N=11) of patients had mutation(MG) and normal homozygote genotype (NHG) had 9.46%(N=7) and 13.51%(N=10) for IL-2 and IL-4 genes, respectively.In patients 2ndgroup the MG(65.15%(N=43) for IL-2 and 69.70%(N=46) for IL-4 gene) was higher than:19.70%(N=13) and 13.64%(N=9) of patients had HeG and NHG had 15.15%(N=10) and 16.67%(N=11) for IL-2 and IL-4 genes, respectively.In contrast, most of healthy donors had NHG with 60.00%(N=18) and low frequency of MG at 16.67%(N=5) and 23.34%(N=7) and HeG at 23.33%(N=7) and 16.67%(N=5) for IL-2 and IL-4genes,respectively(p<0.05). Conclusion.Compared to healthy controls patients with tuberculosis had significantly lower levels of serum IL-4 and high - IL-2. This coincided with greater frequency of HeG in 1group and MG in 2group polymorphisms C-589T and T-330G genes of IL-4 and IL-2. Further studies are warranted whether higher rate of MDRTB has a causal immunogenetic relationship to polymorphism of genes encoding for IL-2 and IL-4 than patients without MDRTB.