The state of oxidative-antioxidant homeostasis under the influence of subtoxic doses of sodium fluoride

Abstract

The objective of the study was to study the state of oxidative-antioxidant homeostasis under the conditions of the formation of experimental fluoride intoxication.24 white rats of the Wistar population were daily injected with sodium fluoride solution (20 mg/kg of animal weight). The continuation of intragastric xenobiotic intake was 1.5 months. The control group was monitored for 2 weeks toanalyse their condition. After 2 weeks, and The study program was conducted after 2 weeks when healthy mature (3 months) males weighing 180-200 grams were selected . At the end of the experiment, the control and experimental groups were clogged by decapitation. Evaluation of oxidative-antioxidant homeostasis was doneon the basis of the following indicators: the intensity of biochemiluminescence (BCL) of homogenates of internal organs and blood serum, the blood levels of SH-groups, glutathione, haptoglobin, diene conjugates (DC), malondialdehyde (MDA), cysteine, catalase activity, peroxidase, ceruloplasmine, glutathione peroxidase, superoxide dismutase. Vitamin C was determined in the adrenal glands. Analyzing the effect of fluoride intoxication on the state of oxidative and antioxidant system, it was shown that stimulation of free radical lipid peroxidation, the accumulation of peroxides, hydroperoxides, free radicals and inhibition of the antioxidant system are characteristic in this process. The obtained results confirm that all these mechanisms underlie the formation of structural and metabolic mechanisms of the pathogenesis of fluoride intoxication.