Наукові праці. Кафедра фізіології

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A study of enterocyte membranes during activation of apoptotic processes in chronic carrageenan-induced gastroenterocolitis
(2018-08) Tkachenko, А.; Marakushyn, Dmytro; Kalashnyk, Yulia; Onishchenko, A.; Gorbach, Tetiana; Nakonechna, O.; Posokhov, Y.; Tsygankov, A.; Korniyenko, Ye.
Aim To investigate the lipid membranes of rat enterocytes in chronic carrageenan-induced gastroenterocolitis accompanied by the activation of apoptotic processes. Methods Steady-state fluorescence spectroscopy: a study by fluorescent probes - by ortho-hydroxy derivatives of 2,5-diaryl-1,3- oxazole. Activity of apoptosis signal-regulating kinase 1 and poly (ADP-ribose) polym erase in small intestinal homogenates, blood serum levels of matrix metalloproteinase-2 and caspase-3 and the level of DNA fragmentation in small intestinal homogenates were determined. Results Biochemical analysis revealed that an activation of apoptotic processes occurred in the intestinal epithelium of rats during chronic carrageenan-induced gastroenterocolitis. The fluorescence probes showed that activation of apoptotic processes in carrageenan-induced gastroenterocolitis was accompanied by changes in polar regions of rat enterocyte membranes, while no changes were revealed in more hydrophobic regions of the membranes. Conclusion The increase in hydration of membranes was attributed to the activation of the apoptosis of enterocytes. It has been shown that a fluorescent probe (2-(2'-hydroxyphenyl)-5-phenyl-l,3-oxa- zole) can be used for the detection of apoptosis of enterocytes.
A study of erythrocyte membranes in carrageenaninduced gastroenterocolitis by method of fluorescent probes
(2018-04) Tkachenko, A.; Marakushyn, Dmytro; Rezunenko, Y.; Onishchenko, A.; Nakonechna, O.; Posokhov, Y.
Objective. The aim of our research was to investigate erythrocyte membranes of rats with carrageenan-induced gastroenterocolitis using fluorescent probes (ortho-hydroxy derivatives of 2,5-diaryloxazole).Materials and methods. Fluorescence of O1O (2 - (2’-OH-phenyl)5-phenyl-1,3-oxazole) and PH7 (2-(2’-OH-phenyl)-phenanthro[9,10]-1,3-oxazole) probes in suspensions of erythrocytes of rats with chronic carrageenan-induced gastroenterocolitis was determined and compared to the control group consisted of intact animals.Results. It has been found that the hydration of erythrocyte membranes increases in rats with carrageenan-induced gastroenterocolitis. The disease is associated with the changes in the polar regions of the membranes, while no changes in the hydrophobic areas of the erythrocyte membranes are observed. Conclusion. The detected changes allow us to make a conclusion that the fluidity of the erythrocyte membranes of the rats with chronic carrageenan-induced gastroenterocolitis increases.
A study of influence of working at the computer on some indexes of the physical health state of students
(2018-09) Gordienko, P.; Pandikidis, N.; Shenher, S.
The purpose of the work is to study the level of physical health, ability to work dynamics during the week, prevalence of Internet addiction and the impact of working at a computer on the students’ health state.
Adipokines and interleukin-17 are the link of regulatory mechanism in kidney
(2018-09-28) Goncharova, Аlina
Adipokines and interleukins (IL) are involved in regulation of renal function but their role in the mechanisms is not clear yet. If the effects of such adipokine as adiponectin in kidney disorders are better defined [1], the effects of visfatin and IL17 are not so well studied.
Aggressive parenting: social, medical and legal aspects
(2024-01) Isaieva, Inna; Isaiev, Arsen; Korobtsova, Nataliia; Nadon, Victoriia; Puchkovska, Iryna
Aggressive parenting is considered as style using threats or different kinds of punishments such as psychological or physical to keep a child under control or pressure to obey. However, the standardized definition of aggressive parenting doesn’t exist and most of interpretations are focused on physical aggression which refers to actions with intention of causing physical pain or injury.
Analysis of heart rate variability as a potential marker of shock factors
(2021-10-28) Pandikidis, N.; Maslova, Natalia; Maslova, Yuliia
Changes in the functional state of the autonomic nervous system are accompanied by various functional states of the heart itself, as well as cardiac and non-cardiac diseases. Was conducted an analysis of the literature of recent years, the importance of analyzing heart rate variability in diagnosing various lesions of the central nervous system, as well as predicting the survival of patients.
Anthropological aspects of creative thinking of a person with type-forming premises on the psychophysiological background
(2018-11-08) Vashchuk, N.; Tishchenko, M.
Актуальностью темы является разработка компетентностных подходов к определению общих способностей и творческого мышления [1,5,6]. Системный подход к процессу творчества трехаспектный, интегрирующие его составляющие: предметная, социальная и личностная [2,4]. Поэтому становление интеллекта необходимо рассматривать как сложный многомерный процесс. В www.iscience.me 123 последнее время в психофизиологии творчества решающую роль приобретает социокультурная детерминация, которая рассматривает развитие знаний о человеке как результат культурно-эволюционного развития [3]. Эволюционная физиология стала определять стиль мышления в психологии. На разных уровнях эволюции увеличивается вариативность психических способностей индивидов, входящих в расширяющиеся системы, а также разновидность проявлений человека в биогенезе, антропогенезе, социогенезе и персоногенезе [7,8]. На современном этапе особую значимость приобретают две исследовательские ориентации. Психофизиология находит новые связи между творческой активностью мозга[1,9].
Application of simulation technologies in teaching of theoretical disciplines in medical university
(2017-05-18) Chernobay, Larysa; Hloba, Nataliia; Isaieva, Inna; Karmazina, Iryna
Modern higher medical education undergoes the process of modernization aimed at creation of necessary conditions for professional training of specialists with high level of competence, creative thinking and ability to make valid responsible decisions in process of their professional activity. Reaching of that aim requires changes of traditional ways of educational and pedagogical interaction between teachers and students and including of modern innovation technologies in training process. Taking into account the specificity of professional activity of future doctors, simulation technologies become one of the most important parts of higher medical education. Application of simulation technologies aimed at imitation of real process of doctor’s work allows teachers to form in students during training process all the theoretical knowledge, practical skills and personal traits necessary for their future professional activity.
Architecture of a functional system of the sagittal balance maintaining
(Polish Physiological Society, 2021-09-15) Goncharova, Alina
To understand the mechanisms of maintaining the sagittal balance within physiological borders, it is important to consider it within the p.K. anokhin’s concept of functional system. Consideration of the structures involved in maintaining the sagittal balance from this point of view will allow us to determine the possible causes of its displacement and methods of its correction.
Aspects of diagnostic criteria in women with luteal insufficiency
(2018-10-06) Tishchenko, A.; Tishchenko, M.
Недостаточность лютеиновой фазы (НЛФ) - является гормональным нарушением, все многообразие которого реализуется на уровне эндометрия. Недостаточная гормонально зависимая структурная и функциональная перестройка эндометрия может приводить к отсутствию или дефектам имплантации. Одним из основных секреторных белков эндометрия является альфа-2-микроглобулин фертильности (АМГФ).
Assessment of the time course of the state of extraocular muscles in printed load with different design parameters
(2018-09-30) Maslova, Natalia
The study deals with evaluation of the time course of the state of extraocular muscles of the visual system in 199 pupils of three age groups in printed load, estimated from the ratio of the interference pattern parameters and the symmetry factor introduced by the author. Adaptation of the visual system to its own state (structural and functional organization) was found to occur in many cases due to asymmetric functioning of extraocular muscles. In long work at a close distance this initial (adaptivecompensatory) asymmetry, providing binocular vision, converges with asymmetry caused by visual stress and fatigue. Thus, the study identified three main ways of adapting to work at close distance. Keywords
Autonomic supply of physical activity in young people with arterial hypotension
(2020-02) Marakushyn, Dmytro; Chernobay, Larysa; Isaieva, Inna; Karmazina, Iryna; Goncharova, Аlina; Shenger, Svitlana; Bulynina, Oksana; Sokol, Olena
Totally 128 young people aged 17–21 were examined including 50 individuals with normal values of blood pressure and 78 people with arterial hypotension. Young people with arterial hypotension were ranged according to the level of arteral pressure: first group included 39 persons (moderate arterial hypotension) where the level of mean arterial pressure was determined as 80–75 mm Hg; the second group (expressed arterial hypotension) included 39 people with level of mean arterial pressure 74.9–70 mm Hg followed by an analysis of the nature of the adaptive capacity of cardiovascular system to exercise and in the recovery period.
Beyond survival: distance learning as a lifeline for medical education in Ukraine during the war
(2024-05-28) Marakushyn, Dmytro; Bondareva, Alla; Morozov, Oleksandr
The war in Ukraine, which has been going on for the second year in a row, could not but affect all areas of life, including higher education. The most important task for universities is not only to ensure the continuity of education, but also to guarantee the safety of students.
Biotechnology production of medium for cultivation and lyophilization of lactic acid bacteria
(Alex Matsyura Publishing, 2018) Gujvinska, S.; Paliy, A.; Dunaieva, Olha; Paliy, A.; Berezhna, N.
The aim of our work was to develop a medium for the cultivation of lactic acid bacteria and to select the optimal protective mediums for the freeze-drying of lactic acid bacteria. The objects of research were cultures of microorganisms Lactobacillus plantarum 7 and Lactobacillus casei 27 isolated, selected and stored in the National Scientific Center Institute of Experimental and Clinical Veterinary Medicine (NSC "IECVM", Kharkiv). Cultivation of lactobacilli was carried out on medium MRS and Blaurock for 24-72 hours at the temperature of 37 °C. To develop nutrient media for the cultivation of lactobacilli, the selected components in the main composition of the medium: lactose, sodium phosphate disubstituted, sodium citrate. As components of the protective medium, solutions of sucrose, gelatinase, sodium acetate, and aerosol® were used. Because of the studies, the optimal composition of the nutrient medium was determined (lactose 1.2 %, sodium phosphate disubstituted 0.3 %, and sodium citrate 0.6 %) for the cultivation of lactic acid bacteria on which bacteria grow and actively accumulate a significant number of viable cells from 7.1 to 8.2 × 106 CFU/cm3. In the various cultivation regimes of lactobacilli, pH 7.0 is optimal, and the temperature of the inoculum incubation is 37±0,5 °C. An alternative protective medium is added with the addition of 10 % sucrose, 1.0 % gelatinase and 3.0 % aerosil, which ensures the viability of viable cells after lyophilization of strains of L. plantarum 7 and L. casei 27, respectively, 96 % and 97 %. A culture medium was proposed (lactose 1.2 %, sodium phosphate disubstituted 0.3 %, sodium citrate 0.6 %) for cultivation of lactobacilli on which bacteria grow and actively accumulate a significant number of viable cells (7.1-8.2) × 106 CFU/cm3). Under different temperature regimes and pH of the medium, the best is that it has a pH of 7.0 and the incubation of the inoculum passes at a temperature of 37 ± 0.5 °C. The maximum preservation of viable cells after lyophilization of strains L. plantarum 7 and L. casei 27, respectively, 96 % and 97 % were observed using a protective medium supplemented with 10 % sucrose, 1.0 % gelatose and 3.0 % aerosil. The addition of protective media according to the given formulation with the freeze-drying of lactic acid bacteria and the proposed drying regime have technological advantages and ensure the preservation of the basic biological properties of microorganisms.
Bone remodeling regulation in the model of combined unloading with chronic inflammation in rats
(2021-04-06) Goncharova, Alina
Combined effect of mechanical unloading with induced chronic inflammation results in dysregulation of the mechanisms of bone homeostasis control.
Brain integrated activity in medical students under conditions of adaptation to psychoemotional stress
(2019-03) Marakushyn, Dmytro; Chernobay, Larysa; Vasylieva, O.; Morozov, O.; Korobchanskyi, Pavlo
According to the results of the study the development of adaptation to psychoemotional stress, which is considered as an emotional stress in medical students during the first three years of study, has a dynamic and multifactorial character. The degree of adaptation processes’ manifestation is analyzed and it is shown that it correlates with the intellectual capacity, which is based on the features of the brain integrative activity.
Bronchospasm management
(НФаУ, 2023-03-16) Isaieva, Inna; Karmazina, Iryna; Vakulenko, Alina; Rashchupkina, Zinaida
A bronchospasm is a condition when the smooth muscles of the airways constrict, which results in narrowing of bronchial lumen thus reducing airflow by 15% or more. This limits the amount of inhaled oxygen and exhaled carbon dioxide. Bronchospasm is a common symptom of bronchial asthma and allergies. Among other causes of bronchospasm are the following: chronic obstructive pulmonary disorder, emphysema, chronic bronchitis, lungs and bronchi infections, smoking (including electronic cigarettes), air pollution, smoke inhalation from a fire, allergens of environment (pet dander, dust, pollen), some food chemicals and additives, cleaning chemicals, cold weather, exercise, general anesthesia (causing airways irritation) and some drugs such as blood-thinning medications and NSAIDs (Jeffrey J. Fredberg, 2004). Results of scientific researches have shown that bronchospasm was also associated with atopic rhinitis (Laura Kwiatkowski, Jessica Mitchell, 2016).
Cell Cultured Mycoplasma Agglutinin: New Possibilities for Monitorin
(2019-05-20) Bobrovitska, I.; Dunayeva, H.; Stegniy, B.
Monitoring of Mycoplasma gallisepticum (MG) infection combined with regulatory measures (segregation – rejection – sanitation) is the only effective approach to avoid losses of profit in poultry business, which is still observed worldwide. The main tools for MG monitoring include serum plate agglutination (SPA), hemagglutination-inhibition test (HI) and enzyme-linked immunosorbent assay (ELISA). The SPA is much more universal and quick test for different poultry species comparing to other assays. Here we report the results of our attempt to enhance mycoplasma antigen competence in early-term diagnostics of mycoplasma infection in poultry flocks.
Clinical and genetic peculiarities of vascular manifestations of antiphospholipid syndrome (case report)
(2017-06) Vasylyev, D.; Chernobay, Larysa; Vasylieva, O.; Oliinyk, M.; Vashuk, M.
Pathogenetic mechanisms of the development of antiphospholipid syndrome (APS) are considered in the article, which is the basis for the development of clinical manifestations and laboratory markers of APS. The modern literature data are analyzed, according to which the presence of antiphospholipid antibodies is a hypercoagulable background, and the formation of thrombi occurs under the influence of other allowing procoagulation factors. The classification of the main types of hereditary thrombophilia is given, which is the primary disorder, against the background of which an autoimmune thrombosis APS develops.
Clinical course and public health
(Харківський національний медичний університет, 2018-10-24) Vashuk, M.; Moroz, G.; Hloba, Nataliia
Weakness, fatigue, headache due to weather changes are detected in about 70 % of population and may indirectly or even directly influence the well-being of a person. In its turn, individual health of each of citizens altogether constitute the public health of the whole population [1, p. 119]. And despite the fact that the great compensation mechanisms are hidden within human adaptive abilities, meteosensitivity is one of its flaws during adaptation to changes of environmental parameters. If reserve of adaptive abilities is determining the health level, then manifestation of meteosensitivity proves health being weak. Intensity of meteopathic reactions depends on organism’s state, availability of diseases, nature conditions, social environment, etc. Various scientific sources have wide coverage of interaction between meteosensitivity and physical load [2, p. 142–143], psychic-physiological adaptation and geomagnetic situation [3, p. 26], however, the data about influence of meteorological factors on state of public health and clinical course of illnesses is insufficient, thus determining the importance of current research.