Кафедра біологічної хімії
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Browsing Кафедра біологічної хімії by Subject "2023а"
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Item Effect of digoxin, sodium valproate, and celecoxib on the cerebral cyclooxygenase pathway and neuron-specific enolase under the pentylenetetrazole-induced kindling in mice(2023) Tsyvunin, Vadim; Shtrygol’, Sergiy; Mishchenko, Mariia; Lytkin, Dmytro; Taran, Andriy; Shtrygol’, Diana; Gorbach, TatianaNeuroinflammation plays an important role in the pathogenesis of epilepsy, so it is necessary to clarify the influence of standard antiepileptic drugs as well as adjuvant agents (e.g., cardiac glycoside digoxin, which previously showed a clear аanticonvulsant potential) on cyclooxygenase pathway and neuron-specific enolase under the conditions of chronic epileptogenesis. The aim of the article is to determine the effect of digoxin, sodium valproate, and celecoxib per se, as well as the combination of digoxin with sodium valproate on the content of cyclooxygenase 1 and 2 types, prostaglandins E2, F2α, I2, thromboxane B2, 8-isoprostane and neuron-specific enolase in the brain of mice in the pentylenetetrazole-induced kindling model. It was found that only the combination of sodium valproate with digoxin provides a complete protective effect (absence of seizures) and shows the clearest influence on neuroinflammation markers and neuronal damage than monotherapy with each of these drugs and celecoxib, which appeared to be an ineffective anticonvulsant. The obtained results indicate that digoxin is a promising adjuvant drug to classical antiepileptic drugs (mostly sodium valproate) in epilepsy treatment.Item Exhaled phospholipids as a prognostic factor of pulmonary complications in children with acute lymphoblastic leukemia(2023) Makieieva, N.; Koval, V.; Gorbach, T.Introduction. Pulmonary complications are common in children with acute lymphoblastic leukemia (ALL). The assessment of phospholipids (PL) in the exhaled breath condensate can provide more information about pathological processes in the lungs in children with ALL. Purpose — to assess the level of PL in the exhaled breath condensate (EBC) in children with ALL and its prognostic value. Materials and methods. 40 children with ALL aged 6–17 years were examined. 1st group included newly diagnosed children with ALL (n=18). 2nd group involved ALL survivors who had completed the total course of chemotherapy (n=22). The control (C) group consisted of 15 healthy children. The levels of PL in the EBC were investigated by spectrophotometric thin-layer chromatography using an SPh 46 spectrophotometer. Results. The frequency of pulmonary complication was 82.5% during chemotherapy protocols and 18.4% in ALL survivals. The statistically significant increase in the level of phospholipids in 1st (150.75 (137.62; 158.45) mmol/l) and 2nd (130.12 (120.59; 138.34) mmol/l) ALL groups compared with the group C (54.80 (48.30; 60.80) mmol/l) has been detected (p1-C=0.0000; p2-C=0.0000). Children of the 1st group had significantly higher levels of PL in the EBC than children of the 2nd group (p1-2=0.002911). PL level in EBC collected during induction phase of chemotherapy >132.15 mmol/l can be prognostic for the development of acute pulmonary complications (Sensitivity 93.75%; Specificity 100%). PL level in EBC collected after a complete course of chemotherapy >133.28 mmol/l can be predictive for persistent pulmonary complications (Sensitivity 100.00%; Specificity 83.33%). Conclusions. PL level in EBC can be prognostic for the development of pulmonary complications, both during chemotherapy and in longterm remission after completed chemotherapy course. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.Item Experimental Study of the Effect of GdYVO4 :Eu3 Nanoparticles on the Metabolic and Histologic Features of Liver(Gastro Med Res., 2023) Gubina-Vakulik, G.; Nakonechna, O.; Gorbach, T.; Denisenko, S.; Balak, A.; Klochkov, V.; Yefimova, S.Item Reactive oxygen species generation by blood leucocytes of rats with polycystic ovary syndrome under the conditions of intermittent cold exposure(2023) Zhulikova, Maryna; Myroshnychenko, Mykhailo; Nakonechna, Oksana; Zhulikov, Oleh; Pustova, Nataliia; Bibichenko, Viktoriia; Lytvynenko, Olena; Kucheriavchenko, MarynaThe aim of this study was to determine the level of ROS production by blood leukocytes of rats with PCOS under the conditions of intermittent cold exposure. Materials and methods: In the study, 40 immature female rats of the WAG population at the age of 27 days with a body weight of 80-90 g were used. Five groups were formed (8 animals in each group). Group 1 was represented by intact rats that were not subjected to any manipulations. Group 2 was represented by rats that were injected subcutaneously with 0.2 ml of purified and sterilized olive oil daily for 25 days. Group 3 was represented by rats that were exposed to intermittent cold for 25 days. Group 4 was represented by rats that were modeled with PCOS. Group 5 was represented by rats, which were simulated PCOS against the background of intermittent cold exposure. ROS production was estimated in leukocytes isolated from rats of all groups by flow cytometry using the fluorescent probe of 2,7-dichlorodihydrofluorescein diacetate (H2DCFDA). Results: The experimental study revealed an intracellular excessive production of ROS by leukocytes in rats with polycystic ovary syndrome. The use of inter¬mittent cold exposure normalized the production of reactive oxygen species by leukocytes in rats with polycystic ovary syndrome. Conclusions: The effectiveness of intermittent cold exposure, proven by the authors, allows recommending its use as one of the methods of prevention and treatment of the polycystic ovary syndrome.