Browsing by Author "Malanchuk, Vladislav"
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Item Clinical and morphological correlation dependencies and their significance in patients with complications of labored eruption of the lower third molars(2021-03) Malanchuk, Vladislav; Volovar, Oksana; Oblap, Mykola; Brodetskyi, Igor; Dobryi-Vechir, Tatyana; Hryhorovskyi, Valerii; Brodetska, Liudmyla; Dyadyk, Olena; Myroshnychenko, MykhailoThe aim is to determine the clinical and morphological dependencies, which are important for diagnostics, treatment and prediction of outcomes of pathological processes in the region of the LTM with complicated eruption, as based on the study of histopathological changes of paradental tissue (mucous membrane, walls of retromolar pocket, alveolar bone tissue). The correlation relationships between the patients’ clinical data and the morphological parameters of damage to paradental tissue are weak, multidirectional and uncertain in the majority of combinations (considering the available number of biopsy specimens studied). The close certain positive dependence between the damage of the squamous epithelium and the inflammation activity in the lamina propria mucosae, covering the tooth: in the vast majority of cases, the presence of damaged epithelium (within the biopsy specimen) is associated with the inflammation of high activity, was established as based on correlation relationships between the morphological parameters of damage to paradental tissue. The found pathological changes and the correlations justify surgical tactics on paradental soft and osseous tissues that are directed on the LTM sparing.Item Expression features of human papilloma virus type 16 and anti- Epstein-Barr virus in pleomorphic adenoma, surrounding and intact salivary gland(2021-08) Brodetskyi, Igor; Malanchuk, Vladislav; Dyadyk, Olena; Myroshnychenko, Mykhailo; Kulbashna, Kulbashna; Astapenko, Olena; Feleshtynska, Oksana; Kapustnyk, Nataliia; Kalashnyk-Vakulenko, YuliiaThe aim is to reveal the expression features of MCA to human papilloma virus type 16 and anti-Epstein-Barr virus in the pleomorphic adenoma, surrounding and intact salivary gland. The severity of the expression of these markers in the salivary gland is determined by the histological variant of the tumor (severely expressed in the epithelial variant, moderately expressed in the mixed variant, and minimally expressed in the mesenchymal variant). The immunohistochemical study has shown that the Epstein-Barr virus and, especially, human papilloma virus type 16 can act as exogenous trigger factors involved in the development of pleomorphic adenoma of the salivary glands. The revealed immunohistochemical features of MCA expression to human papilloma virus type 16 and anti- Epstein-Barr virus in the salivary gland surrounding the pleomorphic adenoma and in the intact tissue of the salivary gland make it possible to recommend the extracapsular dissection of the tumor with resection of the adjacent intact tissue of the salivary gland at a distance of 10 mm in patients with pleomorphic adenoma.Item Histointegration and other terms in dental implantation(2022-03-03) Malanchuk, Vladislav; Klymentiev, Vadym; Myroshnychenko, MykhailoThe article describes biological and non-biological factors that affect the long-term stay of the implant in the bone under functional load, quality indicators of artificial teeth implantation. Instead of the term «osseointegration», the authors use the terms «histointegration» and «histodisintegration» defining them. The following concepts are used: time of histointegration achievement, histofunctional and histoaesthetic integration/disintegration, mechanical and biological stability/destability, integration/disintegration of implant and prosthetic structure, morphological and functional stability and destability of the implant.Item Human papilloma virus type 16 expression in pleomorphic adenomas and adjacent tissue of salivary glands(2021-08) Myroshnychenko, Mykhailo; Brodetskyi, Igor; Dyadyk, Olena; Malanchuk, VladislavItem Immunohistochemical features of the expression of human papilloma virus type 16 in pleomorphic adenomas of salivary gland(2021) Brodetskyi, Igor; Dyadyk, Olena; Malanchuk, Vladislav; Myroshnychenko, Mykhailo; Krotevych, MykhailoHuman papilloma viruses belong to the Papillomaviridae family. Papillomaviruses are one of the most heterogeneous groups of viruses that infect humans and animals. To date, more than 250 papilloma virus types have been identified, and each of these genotypes are associated with infection at particular anatomical sites. Human papilloma viruses, due to differences in DNA sequence, are divided into alpha-, beta-, gamma-, mu- and nu-groups. Human papilloma viruses, taking into account the risk of developing malignant tumors, are classified into viruses of high carcinogenic risk.The severity of the immunohistochemical reaction with amonoclonal antibody to human papilloma virus type 16 depends on the histological variant of the pleomorphic adenoma of the salivary gland.Item Influence of the patient`s sex and age, volumes of the salivary gland and pleomorphic adenoma on the tumor histological variants(2023) Brodetskyi, Igor; Malanchuk, Vladislav; Myroshnychenko, Mykhailo; Riebienkov, Stanislav; Arseniev, Oleksandr; Kotenko, Oleksandr; Brodetska, LiudmylaAim: The purpose of the study was to identify the influence of the patient’s sex and age, the volumes of the salivary gland and pleomorphic adenoma on the histological variants of the tumor. Materials and Methods: The study included 21 women and 9 men with pleomorphic adenomas of the salivary gland. In 15 cases, a mesenchymal variant of pleomorphic adenoma was identified, in 5 cases – an epithelial variant, in 10 cases – a mixed variant. The average age of the patients was 39.7±2.9 years. Patients with pleomorphic adenomas underwent magnetic resonance imaging using a Siemens MAGNETOM Aera 1.5T device (Germany), during which three projections of the tumor and salivary gland were measured (antero-posterior, lateral, vertical) with subsequent calculation of their volumes, and then the ratios of these volumes. Classification trees were used to determine whether patients belonged to one of three variants of pleomorphic adenoma. The CART (Classification And Regression Tree) algorithm was used as a branching option. Stopping branching was carried out using the FACT (Fact-style direct stopping) method until each terminal (final) node of the tree does not contain incorrectly classified observations or when their number becomes less than a given proportion of the total group size (less than 5%). Determination of the structure and relationships between the tumor variants and such indicators as gender, age and radiological indicators (volume of the tumor, volume of the salivary gland, ratio of tumor volume to salivary gland volume) was carried out using correspondence analysis. Results: Epithelial variant of pleomorphic adenoma is more typical for women, and the mixed and mesenchymal variant is more common for patients of both sexes. The epithelial variant can develop in patients of any age, while the mixed variant occurs mainly in patients older than 41 years, and the mesenchymal variant – mainly in patients younger than 41 years. The ratio of the volume of the tumor to the volume of the salivary gland will be predominantly >0.17 in the epithelial variant of pleomorphic adenoma, predominantly ≤0.17 in the mesenchymal variant, and can take any value in the mixed variant. Conclusions: The authors conducted a study in order to identify the influence of sex, age of the patient, and the ratio of the volume of pleomorphic adenoma to the volume of the salivary gland on the histological variant of the tumor. The revealed data will be useful in the treatment and diagnostic process in patients with pleomorphic adenoma of the salivary gland.Item An integrated approach to the morphological diagnosis of different types of pleomorphic adenomas of the salivary gland: long-term research results(2022-07-07) Myroshnychenko, Mykhailo; Brodetskyi, Igor; Malanchuk, Vladislav; Dyadyk, Olena; Arseniev, Oleksandr; Kulbashna, Yaroslava; Astapenko, Olena; Brodetska, Liudmyla; Brodetskyi, Sergey; Bibichenko, ViktoriiaIntroduction. Morphological research methods play an important role in the diagnosis of pleomorphic adenoma in the preoperative and postoperative periods. Pleomorphic adenoma is histologically extremely heterogenous and has a complex structure, which sometimes causes certain difficulties and misdiagnosis for pathologists in the morphological diagnosis of this tumor and its histological variant determination. Pathologists emphasize that pleomorphic adenoma may be confused with myoepithelioma, adenoid cystic carcinoma, mucoepidermoid carcinoma, basal cell adenoma because of its varied histopathological presentation. The results of our own long-term research allow us to identify certain morphological features of various pleomorphic adenomas and, on their basis, to formulate an integrated approach to the morphological diagnosis of these tumor variants. The aim is to describe an integrated approach to the morphological diagnosis of different types of pleomorphic adenomas of the salivary gland. Materials and methods. Surgical and biopsy material from 30 patients with pleomorphic adenomas of epithelial, mixed and mesenchymal variants was studied using histological, immunohistochemical, genetic, morphometric and statistical methods. Results. The results of research allowed us to identify methods for determination the pleomorphic adenomas types. The first method requires an immunohistochemical reaction with a monoclonal antibody to human papillomavirus type 16, followed by counting the percentage of positively stained cells in the tumor. Thus, the mesenchymal variant of the tumor is diagnosed when the percentage of positively stained cells is < 40%. In the mixed variant, this indicator is ≥ 40%, but ≤ 70%, and in epithelial variant – > 70%. The second method was based on the multivariate discriminant analysis. Three formulae were derived to determine the tumor types (Fmesenchymal = - 41.03 + 4.96Х1 + 1.11Х2, Fepithelial = - 22.27 + 3.46Х1 + 0.85Х2, Fmixed = - 122.25 + 5.63Х1 + 3.2Х2, here Х1 - number of vessels, Х2 – specific volume of parenchyma). Conclusions. The authors identified several methods for determining the histological variants of pleomorphic adenomas. These methods will improve the morphological diagnosis of pleomorphic adenomas variants in the preoperative and postoperative periods.Item Morphological changes in major salivary glands tissue, surrounding the pleomorphic adenomas(2022-09-27) Brodetskyi, Igor; Malanchuk, Vladislav; Myroshnychenko, MykhailoItem Morphological characteristics of reparative osteogenesis in the rats lower jaw under the conditions of using electrical stimulation(2023) Huseynov, Agil; Malanchuk, Vladislav; Myroshnychenko, Mykhailo; Markovska, Olena; Sukharieva, Liliia; Kuznetsova, MilenaAim: The purpose of the study was to identify the morphological features of reparative osteogenesis in the rats lower jaw under the conditions of using electrical stimulation. Materials and Methods: An experiment was conducted on 24 mature male rats of the WAG population. Two groups were formed. Group 1 included 12 rats that were modeled with a perforated defect of the lower jaw body. Group 2 included 12 animals that were modeled with a perforated defect similar to group 1. In animals, a microdevice for electrical action was implanted subcutaneously in the neck area on the side of the simulated bone defect (a temporary Videx AG 4 battery; a constant sinusoidal electric current of an unchanging nature 1 milliampere, frequency 30 W). The negative electrode connected to the negative pole of the battery was in contact with the bone defect. The battery and electrode were insulated with plastic heat shrink material. Morphological and statistical methods were used. Results: The positive effect of electrical stimulation on reparative osteogenesis was due to a decrease in the severity of hemodynamic disorders, activation of angiogenesis in granulation tissue, which was one of the components of the regenerate that filled the bone defect, matured and turned into connective tissue; stimulation of the proliferative potential of fibroblastic cells and cells with osteoblastic activity in granulation tissue; increasing the proliferative potential of osteoblastic elements of bone tissue bordering the cavity; stimulation of macrophage cells and processes of cleansing the bone cavity from fragments of a blood clot and alteratively changed tissues; formation of clusters of adipocytes in the loci of connective and granulation tissue of the regenerate; the process of metaplasia of connective tissue into bone tissue; an increase of the foci of hematopoiesis in the intertrabecular spaces of lamellar bone tissue. Conclusions: A comprehensive clinical and experimental study conducted by the authors proved that electrical stimulation activates the reparative osteogenesis in the lower jaw, which occurs through direct osteogenesis and does not finish on the 28th day of the experiment. Мета: Метою дослідження було виявити морфологічні особливості репаративного остеогенезу нижньої щелепи щурів за умов застосування електростимуляції. Матеріали і методи: Експеримент проведено на 24 статевозрілих щурах-самцях популяції WAG. Було сформовано дві групи. До 1 групи увійшли 12 щурів, яким моделювали дірчастий дефект тіла нижньої щелепи. 2 група включала 12 тварин, яким моделювали перфорований дефект, подібний до групи 1. Тваринам імплантували мікропристрій для електричної дії підшкірно в ділянку шиї з боку змодельованого дефекту кістки (тимчасовий акумулятор Videx AG 4; постійний синусоїдальний електричний струм незмінної природи 1 міліампер, частота 30 Вт). Негативний електрод, підключений до негативного полюса батареї, контактував з дефектом кістки. Акумулятор і електрод були ізольовані пластиковим термоусадковим матеріалом. Використовувалися морфологічні та статистичні методи. Результати: Позитивний вплив електростимуляції на репаративний остеогенез зумовлений зменшенням вираженості гемодинамічних розладів, активацією ангіогенезу в грануляційній тканині, яка була одним із компонентів регенерату, що заповнював кістковий дефект, дозрівав і перетворювався на сполучну тканину; стимуляція проліферативного потенціалу фібробластичних клітин та клітин з остеобластною активністю в грануляційній тканині; підвищення проліферативного потенціалу остеобластичних елементів кісткової тканини, що межує з порожниною; стимуляція клітин макрофагів і процесів очищення порожнини кістки від фрагментів тромбу і альтеративно змінені тканини; утворення скупчень адипоцитів у вогнищах сполучної та грануляційної тканини регенерату; процес метаплазії сполучної тканини в кісткову; збільшення вогнищ кровотворення в міжтрабекулярних просторах пластинчастої кісткової тканини. Висновки: Комплексне клінічне та експериментальне дослідження проведене авторами довело, що електрична стимуляція активує репаративний остеогенез нижньої щелепи, який відбувається через прямий остеогенез і не завершується на 28 день експерименту.Item Morphological justification of the salivary gland tissue resection boundaries during surgical treatment of patients with pleomorphic adenomas(2021-09) Brodetskyi, Igor; Malanchuk, Vladislav; Dyadyk, Olena; Kulbashna, Yaroslava; Astapenko, Olena; Kapustnyk, Nataliia; Myroshnychenko, MykhailoThe aim is to substantiate morphologically the resection boundaries of the salivary gland tissue in the surgical treatment of patients with pleomorphic adenoma of different histological variants. These changes were: violation of the ratio of the specific volumes of the parenchyma and stroma; atrophy of the terminal sections and ducts with cystic expansion of some ducts; thickening of the secretion and formation of calculi in the lumen of some ducts; atrophic and alterative changes in the epithelial lining the terminal sections and ducts; sclerosis and lipomatosis, areas with hyalinosis and dystrophic calcification in the stroma; hemodynamic disturbances in the stroma with a decrease in the number of vessels; pronounced focal or diffuse immune infiltration in the stroma in some areas with the lymphoid follicles formation.The comprehensive study has confirmed that removal of the tumor with the adjacent tissue of the salivary gland at a distance of 1.0 cm in patients with pleomorphic adenoma of various histological variants is the most justified from the morphological point of view.Item The relationship of clinical and morphological data in comminuted fractures of the lower jaw(2022) Huseynov, Agil; Malanchuk, Vladislav; Grygorovskiy, Valeriy; Brodetskyi, Igor; Myroshnychenko, Mykhailo; Kalashnyk-Vakulenko, YuliiaThe aim is to establish the relationship between some clinical, physiological and morphological parameters in patients with the consequences of an acute injury considering the features of pathological and reparative changes in the bone fragments of the lower jaw formed during a fracture using clinical and morphological methods. Materials and methods: The material of the study was bone fragments of the lower jaw, removed during osteosynthesis operations in 20 patients with traumatic comminuted fractures. Also, the material of the study was the data obtained during the examination of the above patients. Morphological parameters characterizing the state of the fragments tissues removed during surgical treatment of fractures of the lower jaw and clinical parameters characterizing the patient’s condition in cases of traumatic fractures of the lower jaw treatment were identified for frequency and correlation analysis. Different of frequency of individual indicators’ gradations cases was determined by the χ-square test, correlations between parameters (correlations of pairs of parameters “clinic – morphology”, “clinical and laboratory data – morphology”) – by calculating the Pearson association coefficient. Results: The main pathological and reparative changes are determined in the bone fragments of the lower jaw after a traumatic fracture: osteomedullary ischemic necrosis (traumatic bone infarction), nonspecific productive inflammation, endosteal and periosteal bone regenerates. The severity and frequency of pathological and reparative changes in the bone fragments of the lower jaw vary, leading to various correlations between clinical and morphological indicators of the fragment tissues conditions. Reliable correlations were established between individual clinical and morphological indicators of the state of bone fragments corresponding to the values of the association coefficient in the range of 0.3-0.7, in particular: in the pair “age of the patient” – “endosteal regenerates” – the dependence is negative, of medium strength, significant (p<0.01). Conclusions: A comprehensive clinical and morphological examination made it possible to calculate quantitative data that characterize the frequency parameters of the occurrence of certain pathological changes in the bone fragments of the lower jaw after a fracture and the features of individual clinical and morphological parameters conjugation in patients with the consequences of a mechanical jaw injury. The obtained data on the correlation dependences of the type “clinic – morphology” can be used to predict the severity of morphological parameters according to the known values of clinical parameters in patients with the consequences of mandibular fractures.Item Special at-rich sequence-binding protein 2 and its role in healing of the experimental mandible bone tissue defect filling with a synthetic bone graft material and electrical stimulation impact(2024) Huseynov, Agil; Malanchuk, Vladislav; Myroshnychenko, Mykhailo; Kapustnyk, Nataliia; Sukharieva, Liliia; Selivanova, LarisaAim: The purpose of the study was to identify the role of SATB2 in healing of the experimental mandible bone tissue defect filling with a synthetic bone graft material and electrical stimulation impact. Materials and Methods: An experiment was carried out on 48 mature male rats of the WAG population, which were divided into 4 groups. Each group included 12 experimental animals. Group 1 included rats that were modeled with a perforated defect of the lower jaw body. Group 2 included animals that were modeled with a perforated defect similar to group 1. In animals, a microdevice for electrical action was implanted subcutaneously in the neck area on the side of the simulated bone defect. The negative electrode connected to the negative pole of the battery was in contact with the bone defect. The battery and electrode were insulated with plastic heat shrink material. Group 3 included rats that were modeled with a perforated defect similar to previous groups, the cavity of which was filled with synthetic bone graft “Biomin GT” (RAPID, Ukraine). Group 4 included animals that were modeled with a perforated defect similar to groups 1-3, the cavity of which was filled with synthetic bone graft “Biomin GT” (RAPID, Ukraine). The simulation of electrical stimulation was the same as in group 2. The material for the morphological study was a fragment of the body of the lower jaw from the zone of the perforated defect. Immunohistochemical study was performed using rabbit anti-human SATB2 monoclonal antibody. Results: In the regenerate filling the defect in the bone tissue of the lower jaw of rats, there was an increase in SATB2 expression under conditions of electrical stimulation; filling the defect with a synthetic bone graft material; simultaneous filling the defect with a synthetic bone graft material and electrical stimulation. The most pronounced expression of SATB2 was observed under conditions of simultaneous filling the defect with a synthetic bone graft material and electrical stimulation; minimally expressed – in conditions of filling the defect with a synthetic bone graft material; moderately expressed – under conditions of electrical stimulation. In the regenerate, in cases of all treatment methods, SATB2 was expressed by immune cells, fibroblastic differon cells, osteoblasts, and in case of electrical stimulation, also by adipocytes, vascular pericytes and endothelial cells, epidermis. Conclusions: The activation of SATB2 expression identified by the authors is one of the mechanisms for stimulating reparative osteogenesis under the conditions of electrical stimulation; filling the defect with a synthetic bone graft material; simultaneous filling the defect with a synthetic bone graft material and electrical stimulation. Мета: Мета дослідження – визначити роль SATB2 у загоєнні експериментального дефекту кісткової тканини нижньої щелепи, заповненому синтетичним кістковим трансплантатом та електростимуляційним впливом. Матеріали і методи: Експеримент проведено на 48 статевозрілих щурах-самцях популяції WAG, які були поділені на 4 групи. Кожна група включала 12 піддослідних тварин. До 1 групи увійшли щури, яким моделювався перфорований дефект тіла нижньої щелепи. До групи 2 увійшли тварини, яким моделювали перфораційний дефект, подібний до групи 1. Тваринам імплантували мікропристрій для електричної дії підшкірно в ділянку шиї з боку імітованого дефекту кістки. Негативний електрод, підключений до негативного полюса батареї, контактував з дефектом кістки. Акумулятор і електрод були ізольовані пластиковим термоусадковим матеріалом. 3 група включала щурів, яким моделювали перфорований дефект, подібний до попередніх груп, порожнину якого заповнювали синтетичним кістковим трансплантатом «Біомін ГТ» (РАПІД, Україна). До 4 групи увійшли тварини, яким моделювався перфорований дефект, подібний до груп 1-3, порожнину якого заповнювали синтетичним кістковим трансплантатом «Біомін ГТ» (РАПІД, Україна). Моделювання електростимуляції проводилося так само, як і в 2 групі. Матеріалом для морфологічного дослідження був фрагмент тіла нижньої щелепи із зони дірчастого дефекту. Імуногістохімічне дослідження проводили з використанням кролячих моноклональних антитіл проти людини SATB2. Результати: При заповненні регенератом дефекту кісткової тканини нижньої щелепи щурів спостерігалося підвищення експресії SATB2 в умовах електростимуляції; заповнення дефекту синтетичним кістково-трансплантаційним матеріалом; одночасне заповнення дефекту синтетичним кістковим трансплантатом і електростимуляція. Найбільш виражена експресія SATB2 спостерігалась за умов одночасного заповнення дефекту синтетичним кістковим трансплантатом та електростимуляції; мінімально виражені – в умовах заповнення дефекту синтетичним кістково-трансплантаційним матеріалом; помірно виражені – в умовах електростимуляції. У регенераті при всіх методах лікування SATB2 експресувався імунними клітинами, фібробластичними дифероновими клітинами, остеобластами, а при електричній стимуляції також адипоцитами, судинними перицитами та ендотеліальними клітинами епідермісу. Висновки: Виявлена авторами активація експресії SATB2 є одним із механізмів стимуляції репаративного остеогенезу в умовах електростимуляції; заповнення дефекту синтетичним кістково-трансплантаційним матеріалом; одночасне заповнення дефекту синтетичним кістковим трансплантатом і електростимуляція.Item Tumors and tumor-like lesions of the salivary glands: morphological characteristics of the surgical material(2021-04) Brodetskyi, Igor; Malanchuk, Vladislav; Sorokin, Bogdan; Myroshnychenko, Mykhailo; Beketova, Yuliya; Dyadyk, Olena; Kapustnyk, Nataliia; Krotevych, Mykhailo; Brodetskyi, SergeyThe aim is to give a morphological characteristic of tumors and tumor-like lesions of the salivary glands in the course of a comprehensive analysis of the surgical material. The connective tissue capsule separating the tumor tissue from the intact tissue of the salivary gland was clearly pronounced in adenolymphomas, and in pleomorphic adenomas it could be clearly expressed, intact, with tumor invasion or absent. In mesenchymal and mixed variants of pleomorphic adenomas, in comparison with the epithelial variant, there was a frequent tumor invasion of the capsule, thinning of the capsule or its absence. Morphological examination of the surgical material allows us to diagnose tumors (pleomorphic adenomas and adenolymphomas) and non-tumor lesions (cysts) of the salivary glands. This, of course, is of great therapeutic, diagnostic and prognostic value. Among the identified pathology of the salivary glands, pleomorphic adenomas cause certain difficulties in morphological diagnosis due to their structural diversity and heterogeneity, sometimes requiring morphometric, immunohistochemical or genetic research.Item Кeratinizing pleomorphic adenoma of parotid salivary glands: analysis of three cases from practice(2022-09-07) Myroshnychenko, Mykhailo; Brodetskyi, Igor; Malanchuk, Vladislav; Dyadyk, Olena; Kalashnyk-Vakulenko, Yuliia