Chronic inflammatory process of the genitourinary system in WAG rats population development of own modeling methodology

Abstract

Experimental models of the urogenital chronic infection process are keys to studying the mechanisms of the pathogenesis of development of kidneys pathology in the offspring, which is associated with the infection of women during pregnancy or even before pregnancy with the determination of the trigger factors for the development of the inflammatory process. Aim. The aim of research was to develop an experimental model of genitourinary system chronic inflammatory process in sexually mature female rats of the WAG population, caused by Proteus mirabilis and Streptococcus pyogenes, taking into account the ability to form biofilms, as a leading factor in the pathogenicity of these pathogens. Material and methods. 8 months aged sexually mature female rats of the WAG population weighing 200-300 grams were divided into three groups for the experiment. Microbiological and morphological methods were used. The material for morphological research was the kidneys of rats from the control and experimental groups. The morphometric method was used to objectify the results of the study. The width of nephrogenic zone of cortical layer in newborn rats, the width of cortical layer and the numerical density of glomeruli in all age categories were determined in kidney preparations stained with hematoxylin and eosin by videomicroscopic morphometry using the Olympus DP-Soft program (Version 3:1) and the produced micropreparations were examined using ZEISS Primostar 3 microscopes (Carl Zeiss, Germany) with a built-in color digital camera, BRESSER Science TFM-301 Trino with a BRESSER Full HD camera (Bresser GmbH, Germany). Results. The chronic inflammatory process of the genitourinary system was reproduced by transurethral and vaginal administration of a daily biofilm form of Proteus mirabilis uroisolate (I group of rats) and Streptococcus pyogenes uroisolate (II group of rats) in 0.2 ml once a day three days in a row. On 10th day there was once injected intraperitoneally reference strains of Proteus mirabilis in group I rats, and Streptococcus pyogenes in group II rats. On the 19th-22nd day after planting the male rats, the female rats gave birth to offspring. In micropreparations of genitourinary system organs of both groups rats general pathological inflammatory processes of various nature were found: morphological study revealed vaginitis, cervicitis, endometritis and myometritis, oophoritis in chronic form together with chronic urethritis, chronic cystitis and chronic pyelonephritis. Conclusions. This original method of modeling of genitourinary system chronic inflammatory process should be used when studying the development of pathological processes in offspring born from mothers with pathology of urogenital tract.