Phenolic compound composition of herb of pulsatilla pratensis (L.) mill

Abstract

The manuscript presents the results of the determination of phenolic compounds in the herb of meadow pasqueflower. Determination of phenolic compounds has been performed by the high performance liquid chromatography (HPLC). Identification of separated substances was performed by the comparison of the retention time of main peaks in the chromatogram of meadow pasqueflower herb with the retention time of standard substances and by reference of UV-spectra with spectra of standard substances like quercetin, hyperoside, luteolin, rutin, apigenin-7-glucoside, caffeic and ferulic acid, umbelliferone and scopoletin. 9 phenolic compounds were identified in the meadow pasqueflower herb using HPLC. Such flavanoids as rutin, hyperoside, apigenin-7-glucoside, luteolin and quercetin were identified and quantified in the herb of meadow pasqueflower. Hyperoside (0.02437 %) and luteolin (0.01568 %) are found in higher quantity. Content of hydroxycinnamic acids, such as caffeic and ferulic acids, is 0.00257 % and 0.00228 %, respectively. Coumarins like umbelliferone (0.00770 %) and scopoletin (0.00037 %) were also found.