Study of pathogenic factors of E.coli isolated from patients with peritonitis

Abstract

The aim: To study the biological properties of museum and clinical strains of E.coliisolated from patients with peritonitis. Materials and methods: It was used 94 strains (clinical, museum and reference). The ability of E. colito adhere was investigated by hemadhezive method to formal human erythrocytes of 0 (I) Rh-positive blood group. The study measured the ability of microorganisms to produce gelatinaze, caseinase, fjbrinolysin, hemolysin. To control of the enzyme activity the positive and negative control with reference strains were used. Synchronisation of cultures activity before experiments was achieved by one-time efgect of low temperature (+4 С) during 30 minutes. Results: To investigate the pathogenic factors of E. coliwe carried out determining of proteolytic, gelatinous, caseinous, fjbrinolytic, haemolytic and adhesion properties. Conclusions: In our investigation pathogen icspecies of Esherihies are virtually indistinguishable from representatives of normal microfmora on its morphological, biochemical and cultural properties. During investigation of serological properties of selected of E. colistrains (n = 94) 65.8% of pathogenic serotypes were revealed. Moreover, all marked E. coliisolated from the abdominal cavity of children and adults, as well as museum strains related to enteropathogenic E. coli(O127: K63, O33: K-) and 1 – to enteroinvazive Escherichia coli(O144: K-).