Fatty acid composition and microbial community in urine as detected by gas chromatography – mass spectrometry

Abstract

The presence of different bacteria, protozoa, and intracellular parasites in urogenital organs complicates the study of disbiosis by traditional methods. Gas chromatography-mass spectrometry was used for identification and quantitative estimation of micro-organisms by the presence of chemical compound or microbial metabolites urine. This short-term analysis (5 hours) is universal, does not require precultivation and pure cultures isolation using selective media. Test biological specimens not needed. Local database and programme of marker control was developed for GCMS research based on lipid biomarker analysis and balance equation methodology and PC calculation with the Microsoft Excel Tables.