A study of enterocyte membranes during activation of apoptotic processes in chronic carrageenan-induced gastroenterocolitis
Date
2018-08
Authors
Tkachenko, А.
Marakushyn, Dmytro
Kalashnyk, Yulia
Onishchenko, A.
Gorbach, Tetiana
Nakonechna, O.
Posokhov, Y.
Tsygankov, A.
Korniyenko, Ye.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aim To investigate the lipid membranes of rat enterocytes in chronic carrageenan-induced gastroenterocolitis accompanied by the activation of apoptotic processes.
Methods Steady-state fluorescence spectroscopy: a study by fluorescent probes - by ortho-hydroxy derivatives of 2,5-diaryl-1,3- oxazole. Activity of apoptosis signal-regulating kinase 1 and poly (ADP-ribose) polym erase in small intestinal homogenates, blood serum levels of matrix metalloproteinase-2 and caspase-3 and the level of DNA fragmentation in small intestinal homogenates were determined.
Results Biochemical analysis revealed that an activation of apoptotic processes occurred in the intestinal epithelium of rats during chronic carrageenan-induced gastroenterocolitis. The fluorescence probes showed that activation of apoptotic processes in carrageenan-induced gastroenterocolitis was accompanied by changes in polar regions of rat enterocyte membranes, while no changes were revealed in more hydrophobic regions of the membranes.
Conclusion The increase in hydration of membranes was attributed to the activation of the apoptosis of enterocytes. It has been shown that a fluorescent probe (2-(2'-hydroxyphenyl)-5-phenyl-l,3-oxa- zole) can be used for the detection of apoptosis of enterocytes.
Description
In conclusion, an increase in hydration of the polar regions of enterocyte membranes occurs in rats with chronic carrageenan-induced gastroenteroco- iitis. The increase in hydration has been attributed to the activation of the apoptosis of enterocytes. A fluorescent probe (2-(2'-hydroxyphenyl)- 5-phenyl-l,3-oxazole) could be used for the detection of apoptosis of enterocytes.
Keywords
apoptosis, food additives, caspases, rats
Citation
A study of enterocyte membranes during activation of apoptotic processes in chronic carrageenan-induced gastroenterocolitis / A. Tkachenko, D. Marakushyn, I. Kalashnyk, Y. Korniyenko, A. Onishchenko, T. Gorbach, O. Nakonechna, Y. Posokhov, A. Tsygankov // Medicinski Glasnik. – 2018. – Vol. 15, № 2. – Р. 87–92.