A study of erythrocyte membranes in carrageenaninduced gastroenterocolitis by method of fluorescent probes
Date
2018-04
Authors
Tkachenko, A.
Marakushyn, Dmytro
Rezunenko, Y.
Onishchenko, A.
Nakonechna, O.
Posokhov, Y.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Objective. The aim of our research was to investigate erythrocyte membranes of rats with carrageenan-induced gastroenterocolitis using fluorescent probes (ortho-hydroxy derivatives of 2,5-diaryloxazole).Materials and methods. Fluorescence of O1O (2 - (2’-OH-phenyl)5-phenyl-1,3-oxazole) and PH7 (2-(2’-OH-phenyl)-phenanthro[9,10]-1,3-oxazole) probes in suspensions of erythrocytes of rats with chronic carrageenan-induced gastroenterocolitis was determined and compared to the control group consisted of intact animals.Results. It has been found that the hydration of erythrocyte membranes increases in rats with carrageenan-induced gastroenterocolitis. The disease is associated with the changes in the polar regions of the membranes, while no changes in the hydrophobic areas of the erythrocyte membranes are observed. Conclusion. The detected changes allow us to make a conclusion that the fluidity of the erythrocyte membranes of the rats with chronic carrageenan-induced gastroenterocolitis increases.
Description
The following changes occur in the erythrocyte membranes of experimental animals with carrageenan-induced gastroenterocolitis: the increase in the membrane hydration and the decrease in the viscosity of the lipid bilayer (i.e. the increase in the membrane fluidity). The use of fluorescent probes (ortho-hydroxy oxazole derivatives) allows to assess the type and nature of the damaging effect of carrageenan on the structural components of membranes. Thus, the mentioned method, using ortho-hydroxy oxazole derivatives as fluorescent probes, may be suggested for application in the practical medicine for the study of the negative effects similar to carrageenan food additives on the human body.
Keywords
erythrocytes, biomembrane, carrageenan-induced gastroenterocolitis, fluorescent probe
Citation
A study of erythrocyte membranes in carrageenaninduced gastroenterocolitis by method of fluorescent probes / A. S. Tkachenko, D. I. Marakushyn, Yu. K. Rezunenko, A. I. Onishchenko, O. A. Nakonechna, Y. O. Posokhov // Human & Veterinary Medicine International Journal of the Bioflux Society Bioflux. – 2018. – Vol. 10, № 2. – Р. 37–41.