Browsing by Author "Krasova, N."

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Effect of leptin lep – 2548 g > a (rs7799039) and leptin receptor lepr 223 q > r (rs1137101) gene polymorphic variants on the development of cardiovascular complications in patients with type 2 diabetes mellitus
(2022-12-15) Mamontov, Ivan; Plohotnichenko, O.; Krasova, N.; Kolesnikova, A.; Gorshunska, M.; Pochernyaev, A.; Voropay, T.; Romanova, I.; Karachentsev, Yu.; Роltorak, V.
Investigation of 2548G˃A leptine gene polymorphic variant impact on risk of non-alcoholic fatty liver disease in patients with type 2 diabettes mellitus
(2022-08-01) Караченцев, Юрій Іванович; Кравчун, Нонна Олександрівна; Karachentsev, Yurii Ivanovych; Kravchun, Nonna Oleksandrivna; Tyzhnenko, T.; Misyura, K.; Gorshunska, M.; Pochernyaev, A.; Krasova, N.; Gladkih, A.; Leshchenko, Z.; Fedorova, G.; Plohotnichenko, O.; Hromakovska, O.; Kolesnikova, A.; Jаnsen, E.; Роltorak, V.
Background. It is known that single nucleotide polymorphisms (SNPs) in adipokine genes can influence the development of pathological conditions associated with obesity, type 2 diabetes mellitus (T2D), non-alcoholic fatty liver disease (NAFLD) and their complications. In this study, we aimed to investigate the link between common -2548G > A (rs7799039) promoter variant of the human leptin gene (LEP) with leptin levels in type 2 diabetes patients with non-alcoholic fatty liver diseasese. Materials and methods. 61 patients with T2D aged from 28 to 80 years old (34 men / 27 women, age 56.40 ± 0.62 yrs, diabetes duration 7.72 ± 0.45 yrs, BMI 32.20 ± 0.43 kg/m2, WHR 1,00 ± 0,01, HbA1c 7.80 ± 0.19 %) with varying degrees of glycemic control and overweight, without renal insufficiency and 51 sex and age-match control subjects were examined. Genotyping according to SNP LEP 2548G > A was performed using the polymerase chain reaction method with appropriate primers and HhaI endonuclease. Results. In our study of T2DM patients with NAFLD compared to T2D patients without NAFLD features of dyslipidemia i.e. significant increase in triglycerides (p < 0,001), LDL cholesterol (p < 0,1), lower HDL cholesterol (p < 0.001) were found. Stratification of the diabetic patients in the presence and absence of NAFLD showed more pronounced increase in circulating leptin levels in the presence of NAFLD (84.73 ± 13.80 vs. 52.57 ± 6.86 μg/L, respectively), (p < 0.01), which justifies the feasibility of using this indicator for further needs as a diagnostic parameter of the above complication. In our study in GG carriers genotype of the G2548A LEP gene polymorphic locus type 2 diabetes patients with NAFLD the highest level of leptin was observed (159.15 μg/L), compared to other genotypes. Thus, it can be assumed that the G allele is associated with increased leptin levels in the blood of patients with NAFLD. This study showed that women with type 2 diabetes mellitus carrying the GG genotype with the G-2458A polymorphic variant of the LEP gene have 3.4 times higher leptin levels than men carrying the same genotype (p < 0.03). Conclusions. The data obtained regarding the 2548G > A polymorphic variant of the LEP gene can be used as a basis for personalized prevention and the formation of risk groups for the development of NAFLD
The polymorphism -308 G/A of the TNF gene and metabolic imbalance in patients with type 2 diabetes mellitus and non-alcoholic fatty liver disease, taking into account cardiovascular complications
(ДУ "Інститут проблем ендокринної патології ім. В.Я. Данілевського АМН України", 2023-07) Tyzhnenko, T.; Misiura, K.; Kravchun, N.; Gorshunska, M.; Pochernyaev, A.; Krasova, N.; Gladkih, O.; Leshchenko, Z.; Fedorova, G.; Plohotnichenko, O.; Hromakovska, O.; Kolesnikova, A.; Jаnsen, E.; Karachentsev, Yu.; Роltorak, V.
Non-alcoholic fatty liver disease (NAFLD), the most common form of liver disease, is now recognized as a major public health problem worldwide. Tumor necrosis factor alfa (TNF-α), a member of the TNF/TNFR cytokine family, is an intercellular transmission molecule that has been reported in a wide range of human noninfection diseases. The aim of the study was to determine the circulating levels of TNF-α and the nature of its relationships with the components of insulin resistance, both metabolic and hormonal, in patients with type 2 diabetes; and establishing the nature of cardiovascular complications (taking into account TNF-α gene polymorphism) in the presence and absence of NAFLD. Materials and methods. Case-control study included information about 50 practically healthy people from the city of Kharkiv and the region. The examined population (except control subjects) consisted exclusively of patients with type 2 diabetes mellitus with a long-term existence of the disease against the background of metabolic syndrome, with varying degrees of glycemic control and violations of liver homeostasis in the absence of renal failure. 117 people were selected for analysis: 63 of them with type 2 diabetes in the presence of NAFLD and 54 patients with type 2 diabetes without NAFLD. Genotyping for single nucleotide polymorphism -308 G > A of the TNF-α was performed by the method of polymerase chain reaction with appropriate primers and NcoI endonuclease. Testing of statistical hypotheses was carried out using the odds ratio and χ2 criteria at the significance level of P ≤ 0.05. Results. The contribution of the genetic component to the formation of the predisposition to the development of type 2 diabetes mellitus based on the single-nucleotide polymorphism -308 G > A of the TNFα gene was determined, which makes it possible to consider the carrier of the A allele as a factor of increased risk for the development of type 2 diabetes mellitus. No association of the studied polymorphism with the risk of developing NAFLD was found. The obtained data make it possible to assume that the studied polymorphism -308 G > A of the TNFα gene is more associated with the risk of developing type 2 diabetes, and the occurrence or progression of NAFLD primarily depends on metabolic imbalance, and not on the contribution of the studied polymorphism. Conclusions. Non-alcoholic fatty liver disease is closely related to hormonal and metabolic risk factors and markers of cardiovascular disease and type 2 diabetes and may increase the risk of developing and progressing cardiovascular complications. The contribution of the genetic component to the formation of the predisposition to the development of type 2 diabetes mellitus based on the single-nucleotide polymorphism -308 G > A of the TNFα gene was determined, which makes it possible to consider the carrier of the A allele as a factor of increased risk for the development of type 2 diabetes mellitus.