Browsing by Author "Gargin, V."
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Item Antimicrobial Activity Derivatives 2H-pirano[2,3-c]piridines against Pathogens of Intestinal Yersiniosis(Journal of Pharmacy and Nutrition Sciences, 2021) Ivannik, V.; Torianyk, I.; Moiseienko, T.; Skliar, A.; Yeromenko, R.; Hnatiuk, V.; Podrigalo, L.; Nazaryan, R.; Mikhailenko, Nina; Gargin, V.Background: An important aspect in the treatment of patients with intestinal yersiniosis is the administration of effective antibiotic therapy. Performed research aimed to determine the spectrum and level of antimicrobial activity of 2H-pyrano[2,3-c]pyridine derivatives on the museum and clinical strains of gram-negative microorganisms Yersinia enterocolitica. Methodology: The object of the study was 28 synthetic derivatives of 2H-pyrano[2,3-c]pyridine. The compounds were studied according to their chemical structure. Weused the method of serial dilutions in Muller-Hinton liquid nutrient medium with a museum’s and clinical strains of Y.enterocolitica. Results: Studies indicate the promise of further study of the properties of 2Hpyrono[2,3-c]pyridine to create an effective antimicrobial medicine. According to theresults of studies on action of antimicrobial compounds synthesized on the basis of 2H-pyrano[2,3-с]pyridine derivatives, it was found that the MIC of compounds for all Y. enterocolitica strains was 100.0 μg/ml. The MBCC of most cultures of Yersinia (72.3 %) was 200.0 μg/ml. Compound 2{3} had a pronounced antiyersiniotic activity, the inhibitory effect of which was manifested at a concentration of 25.0 μg/ml. Retarding the growth of most Yersinia strains (95.3%) with a MIC of 50.0 μg/ml, the MIC of compounds ranged from 50.0 to 200.0 μg/ml. After statistical data processing, pyridine derivatives (compounds 2{3} and 3{5}) were identified, possessing an effective bacteriostatic and bactericidal effect on Y. enterocolitica strains. Conclusions: The results of the research showed a high antimicrobial activity of 2Hpyrano[2,3-c]pyridine derivatives. The highest activity against Y. enterocolitica wasfound for 2-N2-arylimino-5-hydroxy-methyl-8-methyl-2H-pyrano[2,3-c]pyridine-3-N1-aricarboxamide derivatives.Item Biometric recognition of personality based on spiral computed tomography data(2021) Nechyporenko, А.; Alekseeva, V.; Nazaryan, R.; Gargin, V.Item Changes in the microbiological parameters of the oral cavity caused by using electronic cigarettes(2021-02-27) Tishchenko, O.; Krivenko, L.; Bondarenko, A.; Gargin, V.Item Detection of bone density of paranasal sinuses in women with different level of sex hormons(2021) Alekseeva, V.; Nazaryan, R.; Muryzina, I.; Gargin, V.; Bondarenko, A.Item Determination of aspergillosis of the female genital organs in women with immunodeficiency(2023) Lytvynenko, M.; Bondarenko, A.; Bondarenko, O.; Gargin, V.Item Diagnostic assessment and treatment of cherubism as prevention of ocular complications(2022) Nazaryan, R.; Mikhailenko, N.; Yaroslavska, Yu.; Bondarenko, A.; Gargin, V.; Lytvynenko, M.; Artiomov, O.; Oliynyk, D.; Tregub, T.Item Effect of electronic cigarettes on oral microbial flora(SET Publisher, 2021-08-13) Popova, T.; Kryvenko, L.; Tishchenko, O.; Nakonechna, O.; Podrigalo, L.; Nessonova, T.; Gargin, V.Background: Despite well-documented adverse effects of tobacco consumption, cigarettes use is still rising and part of this increase is related to the popularization of alternative electronic nicotine delivery devices, such as electronic cigarettes (ECs). The aim of the performed research was to assess the effect of electronic cigarettes aerosol on the oral microbiota, using culture methods. Methodology: 30 ten-week-old WAG rats (female 76-81 g and male 86-94 g) were randomly distributed in two groups, as follows: Group 1 – control animals (n = 10); Group 2 – EC aerosol exposed (n = 20). EC aerosol exposures were carried out by using the Boyarchuck chamber. During the study, the rat oral microbiota were collected four times: at the beginning of the experiment, on the 30th, 60th and 90th days. Microorganisms were identified using standard microbiological techniques. Results: EC exposure to Group 2 rats resulted in a depletion of colonies commensal microbes and a greater incidence of atypical species such as Klebsiella pneumoniae, Acinetobacter lwoffii, Candida albicans compared to Group 1 on day 90. The test of independence between frequency distibution of opportunistic microbes and duration of EC exposure showed a significance for Klebsiella pneumoniae – χ2= 8.017, p=0.0456, Acinetobacter lwoffii – χ2= 36.772, p=0.0001, and Candida albicans – χ2=8.689, p=0.0337. Conclusions: The impact of electronic cigarettes facilitated colonization of the oral cavity by opportunistic bacteria and yeast.Item Features of cerebral injury in papillomavirus infection in HIV-positive patients(2023) Gargin, V.; Bondarenko, A.; Katsapov, D.; Bondarenko, O.; Lytvynenko, M.; Bocharova, T.; Nazaryan, R.Item The impact of smoking on the morphofunctional state of periodontal tissues of young organism(ХНМУ, 2021) Romaniuk, A.; Nazaryan, R.; Zakut, Y.; Popova, T.; Gargin, V.Background. The interrelation of disorders in periodontal tissues with metabolic changes caused, in particular, by a violation of microcirculation, observed namely in smoking but the fact that the effect of smoking on the condition of periodontal tissues at a young age has been studied less than other risk factors deserves special attention. The aim of our study was to experimentally assess the effect of smoking on the morphofunctional state of periodontal tissues in young animals. Materials and methods. Simulation of smoking has been performed with ten-week-old WAG rats using the Boyarchuck chamber. Morphometric studies were performed in the gingival zone which was chosen for morphological interpretation of volumes of specific vascular density in microcirculatory bed, specific density of connective tissue in lamina propria and specific area of tissue with ischemia. The obtained digital data were processed by the methods of variation statistics. Results. Simulation of smoking for four weeks in experimental animals leads to pathological changes in the morphofunctional state of the periodontium with reducing vascular density from 19,44±1,97% to 11,03±1,38 %, increasing area of connective tissue from 18,33±2,71% to 26,49±1,24%, spreading area of tissue with ischemia from 1,14±0,70% to 6,35±1,67%. Conclusions. Morphofunctional changes in the periodontium with damage to the structure of the epithelial membrane and changes in its permeability, microcirculatory disorders, sclerotic changes are a manifestation of the initial inflammatory and dystrophic processes that can lead to persistent chronic pathology.Item Importance of antimicrobial factors for microbiocenosis and local immunity of the oral cavity in children with mucoviscidosis(2022) Khmiz, T.; Tkachenko, M.; Kovalenko, N.; Bocharova, T.; Bondarenko, A.; Shevchuk, V.; Odushkina, N.; Nazaryan, R.; Gargin, V.The involvement of commensals and opportunistic pathogens and the role of protective mechanisms in the development of dental diseases in children with cystic fibrosis require more detailed study. The aim of the study was to determine the ecological characteristics of the oral microbiota and some antimicrobial factors of saliva in children with mucoviscidosis. Materials and methods. The study involved an assessment of oral microbiota as complex ecological system that protects the human body from colonization by pathogenic flora in children with cystic fibrosis. Bacteriological studies have been performed on clinical material from 30 children with mucoviscidosis diagnosed with dental and periodontal diseases. Results. In the microbiological study of plaque microbiota, 70 strains of opportunistic pathogens were isolated in patients with mucoviscidosis. The most significant were alpha-hemolytic Streptococci (40%). The proportion of bacteria of Neisseria genus in patients with cystic fibrosis was lower and amounted to 24.3%. C. albicans fungi were isolated in comparable values (18.5%), S. aureus (8.5%), as well as gram-negative strains of E. aerogenes (4.3%) and E. coli (4.3%) significantly dominated. The results indicate that opportunistic pathogens S. aureus, E. aerogenes and E. coli partially replaced the representatives of the normal oral microbiota alpha-hemolytic streptococci and non-pathogenic species of Neisseria genus in patients with mucoviscidosis. Conclusions. Microbiota of plaque in children with mucoviscidosis is characterized by an expansion of the spectrum of opportunistic pathogens due to Staphylococcus aureus, enterobacteria and C. albicans fungi, which indicates a violation of the microbiocenosis due to reduced mucosal immunity. Mucosal immunity of the oral cavity in children with mucoviscidosis is characterized by a 1.5-fold decrease in lysozyme activity and the level of secretory IgA in the saliva of children.Item Importance of antimicrobial factors for microbiocenosis and local immunity of the oral cavity in children with mucoviscidosis(2022) Khmiz, T.; Tkachenko, M.; Kovalenko, N.; Bocharova, T.; Bondarenko, A.; Shevchuk, V.; Odushkina, N.; Nazaryan, R.; Gargin, V.Item Relationship Between Insulin Resistance and Adrenal Steroids Pattern in Women During Menopausal Transition(2021) Muryzina, I.; Gargin, V.; Alekseva, V.Recognition of rise of adrenal androgens experienced by majority of women during Menopause transition (MT) can explain diversity of female phenotypes at postmenopausal ages. Adrenal androgens may mitigate imminent future fallouts of estradiol decline. Perhaps pattern of adrenal androgens predetermines alterations in female organism after menopause including growing insulin resistance in some of them. Objectives. Our study made an attempt to collate insulin resistance index (HOMA--IR) with status of adrenal steroids in women during MT. Methods. Cross-sectional associations were examined between body mass index (BMI), hip- waist ratio (HWR), HOMA-IR and Serum content of insulin, testosterone (T), sex hormone binding globulin (SHBG), free androgen index (FAI), dehydroepiandrosterone sulfate (DHEAS), androstenedione (Adione), delta-5-androstenediol (Adiol) measured in 315 women of perimenopausal age (age range from 45 to 55 years) with amenorrhea less than 2 years. Results. It turned out that women who fell into two different subsets according to HOMA-IR (either raised HOMA-IR – 1st group, or within physiological range – 2nd group) showed androgen profile distinctive to each group. The main significant difference was fivefold higher level of Adiol in the 2nd group, when the 1st group shows preponderance of Adione (p<0.05). Also patients of the 2nd group proved to have lower FAI, less fasting insulin, a bit higher DHEAS, lower BMI and HWR. Conclusion. High Adiol (evidence of activated delta-5 steroidogenic pathway, ethnicity-specific or inherited) provides the ground for physiological course of MT. Excessive androgen activity (Adione – probably ensued from overactivated delta 4/5 isomerase pushing delta-5 hormones to turn into delta-4 ones; T, low SHBG, higher FAI) may incur insulin resistance, android obesity. Stressful excessive cortisol production devastates resources for DHEAS rise and adrenal androgens.Item The relationship between the condition of the oral cavity and the use of tobacco products in different age groups(2024-05) Moroka, R.; Povaliaiev, V.; Tkachenko, I.; Fomenko, Yu.; Babai, O.; Mikulinska-Rudich, Yu.; Iskorostenska, O.; Borisenko, Ye.; Nazaryan, R.; Gargin, V.Understanding the harmful effects of using tobacco products (cigarettes, electronic cigarettes (e-cigarette) or vape, IQOS, hookah, etc.) by various segments of the population is one of the important ways to improve the condition of the tissues of the oral cavity, since smoking is an important risk factor for the occurrence of chronic destructive periodontal diseases.