Neuroinduced mesenchymal stem cells efficiency for reconstruction of rat sciatic nerve defects

Abstract

Peripheral nerves damage is a frequent pathology with significant socio–economic significance. The aim of research was to study regeneration potential of transplanted biodegradable fibrin matrices filled with neuroinduced mesenchymal stem cells for peripheral nerves damage in rats and evaluate degree of anatomical and functional integrity. The study was carried out on 48 mongrel female rats aged 3–4 months (250±50 g) which were divided into 4 groups. Sciatic nerves of all rats were intersected with formation of 10 mm gap and then reconstituted using autografts, acellular fibrin matrix and fibrin matrix with neuroinduced mesenchymal stem cells. Functional, electrophysiological tests and histological evaluation were performed to analyze functional and anatomical recovery during 2 months after injury. It has been shown that using neuroinduced mesenchymal stem cells for restoration of large size gap and recovery of function of peripheral nerve after its damage is effective method according to functional, electrophysiological, histological results.