Please use this identifier to cite or link to this item:
http://repo.knmu.edu.ua/handle/123456789/29916
Title: | Changes in cell membranes of white blood cells treated with a common food additive E407a |
Authors: | Tkachenko, Anton Onishchenko, Anatolii Posokhov, Yevgen Roshal, Alexander Myasoedov, Valeriy Nakonechna, Oksana |
Keywords: | carrageenan cell membrane fluorescent probe leukocytes processed Eucheuma seaweed |
Issue Date: | 2021 |
Citation: | Changes in cell membranes of white blood cells treated with a common food additive E407a / A. Tkachenko, A. Onishchenko, Y. Posokhov, A. Roshal, V. Myasoedov, O. Nakonechna // Turkish Journal of Biochemistry. – 2021. – Vol. 46 (5). – P. 557–562. |
Abstract: | Objectives: To estimate the state of phospholipid bilayer of rats WBCs exposed to a common food additive E407a, which is used as a thickener and emulsifier, during 4 h using a fluorescent probe − ortho-hydroxy derivative of 2,5-diaryl-1,3-oxazole. Materials and methods: Steady-state fluorescence spectroscopy: a study by the environment-sensitive fluorescent probe − 2-(2′-hydroxy-phenyl)-5-phenyl-1,3-oxazole (probe O1O). Results: Changes are detected in the spectra of the fluorescent probe bound to rat WBCs treated with the solutions of E407a of various concentrations in comparison with the corresponding spectra of the probe incubated with the untreated leukocytes. The decrease in polarity and protondonor ability is observed in the lipid membranes of leukocytes in the region, where the probe locates. Conclusions: Our findings suggest a higher dehydration of leukocyte membranes of rats treated with a common food additive E407a at high concentrations and, thus, indicate that exposure to high doses of E407a leads to the increase in the lipid order (i.e. to decrease in fluidity) of the membranes of rat WBCs. |
URI: | https://repo.knmu.edu.ua/handle/123456789/29916 |
Appears in Collections: | Наукові праці. Науково-дослідний інститут експериментальної та клінічної медицини ХНМУ |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
10.1515_tb-2020-0129.pdf | 608,7 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.