Dynamics of indicators of antioxidant protection in response to the application of sodium poly-(2,5-dihydroxyphenilen)-4-thosulfate acid in experimental acute kidney injury

Abstract

Toxic kidney injury causes a change in the activity of antioxidant defence enzymes. Free radical oxidation of lipids is the most important link in the regulation of the lipid composition of biomembranes of cells, membrane-associated enzymes. Activation of lipid peroxidation causes changes in the functions of biomembranes, cell metabolism, which is one of the links in the pathogenesis of kidney diseases. Activation of processes of lipid peroxidation in the kidneys leads to the manifestation of inflammation, disfunction of cell membranes disrupting the balance of functioning of the prooxidant and antioxidant systems. The study of lipid peroxidation in acute kidney injury can assist in establishing the mechanisms of antioxidant protection in the experiment. The purpose of the study is to establish the effect of the investigated drug PDT-Na on the parameters of free radical lipid oxidation and the system of antioxidant defence of the kidneys in rats on models of acute renal failure and gentamicin nephropathy. The studies were conducted on 96 white mature, full-grown non-linear rats of both sexes weighing 150-170 grams. The animals were divided into 16 groups (5 groups per model and one intact group): the animals of the experimental, reference and control pathology groups (1) received the corresponding nephrotoxin. In each model the groups of rats received the following treatment: experimental (2) – PDT-Na, reference – mexidol (3), hofitol – (4) and thiotriazolin (5). The results of the study show that under the conditions of acute kidney injury the PDT-Na assists to reduce the primary intermediates – diene conjugates and secondary intermediates of lipid peroxidation - TBA-reactive products, activation of enzymes of catalase antioxidant defence and superoxide dismutase and accumulation to the almost intact level of GSH endogenous antioxidant. The abovementioned information indicates that the drug of the pharmacological group – antihypoxicant PDT-Na with previously established nephroprotective activity has shown antioxidant, antiradical and cytoprotective properties during the experiment. This is a promising drug for complex treatment of kidney diseases.