Syrup amkesol reduces the level of primary and secondary products of lipid peroxidation and toxic metabolites of nitric oxide in blood serum of immature rats with bronchiolitis
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Abstract
Aim of this study was to determine the pharmacological
effect of syrup Amkesol evaluating the blood serum
levels of primary and secondary products of lipid
peroxidation, activity of AO enzymes and final rates of
NO metabolites in immature rats of different ages with
experimental model of bronchoalveolitis. Materials
and Methods: The study was carried out on 90 WAG
immature rats of ages 1, 2 and 3 menthes, that
correlates by morpho-functional features to 4, 10 and 14
years of human age respectively, on a model of
bronchoalveolitis. Experimental animals in each age
series were randomly divided into 5 groups (n = 6):
intact (healthy), 2 groups of control (untreated with
bronchoalveolitis) 7 and 14 days, and two groups with
bronchoalveolatis that received S-AKS daily during 7
and 14 days. The pathological process implemented by
inhalation of irritant (Sephadex A-25 Pharmacia,
Sweden (5 mg/kg)). In the blood serum samples activity
of CAT, SOD, content of DC, TBARS, total NO,
nitrates and nitrites were determined. Probability of the
results was evaluated by using GraphPad Prism
Software. The critical level of significance was taken
equal to 0.05. Results. The use of S-AKS on the 7th day
in the group of 1- and 3-month-old rats significantly
exceeded activity of CAT compared to the control group
of animals of corresponded age. The SOD activity in
group of 2-month-old animals was restored to intact
level, the activity of CAT exceeded the baseline level
and amounted to 129% (P≤0,05). The concentration of
DC in 1-month-old rats was lower by 45.3% in 2
monthly - by 12.5% than in the control group, in the
group of 3 month-old animals - restored to normal
(P≤0,05). The level of NO metabolites was significantly
decreased compared to the corresponding control group
in all age series (P≤0,05). After 14 days of treatment
with S-AKS in all age group of animals observed
restore the contents of primary and secondary products
of lipid peroxidation compared to the intact level.
Conclusion. Administration of S-AKS reduced the
values of primary and secondary products of lipid
peroxidation; restored the activity of antioxidant
enzymes; reduced the level of NO and its stable
metabolites to the physiological level in all studied age
groups of animals with experimental bronchoalveolitis.
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Storozhenko K. V. Syrup amkesol reduces the level of primary and secondary products of lipid peroxidation and toxic metabolites of nitric oxide in blood serum of immature rats with bronchiolitis / K. V. Storozhenko // Annals of Mechnikov Institute. – 2017. – № 1. – Р. 54–61. – Access mode: http://www.imiamn.org.ua/journal/1_2017/PDF/10.pdf.