VOLUME LXXIV, ISSUE 9 PART 1, SEPTEMBER 2021 Since 1928 ALUNA Publishing House Memory of dr Władysław BiegańskiOfficial journal of the Polish Medical Association Wiadomości Lekarskie monthly journal You can order the subscription for the journal from Wydawnictwo Aluna by: prenumerata@wydawnictwo-aluna.pl Wydawnictwo Aluna Z.M. Przesmyckiego 29 05-510 Konstancin-Jeziorna Poland Place a written order first. If you need, ask for an invoice. Payment should be done to the following account of the Publisher: account number for Polish customers (PLN): 82 1940 1076 3010 7407 0000 0000 Credit Agricole Bank Polska S. A., SWIFT: AGRIPLPR account number for foreign customers (EURO): 57 2490 0005 0000 4600 7604 3035 Alior Bank S. A.: SWIFT: ALBPPLPW Subscription of twelve consecutive issues (1-12): Customers in Poland: 360 PLN/year Customers from other countries: 320 EURO/year Wiadomości Lekarskie is abstracted and indexed in: PUBMED/MEDLINE, SCOPUS, EMBASE, INDEX COPERNICUS, POLISH MINISTRY OF EDUCATION AND SCIENCE, POLISH MEDICAL BIBLIOGRAPHY Copyright: © ALUNA Publishing House. Articles published on-line and available in open access are published under Creative Com- mon Attribution-Non Commercial-No Derivatives 4.0 International (CC BY-NC-ND 4.0) allowing to download articles and share them with others as long as they credit the authors and the pu- blisher, but without permission to change them in any way or use them commercially. Editor in-Chief: Prof. Władysław Pierzchała Deputy Editor in-Chief: Prof. Aleksander Sieroń Statistical Editor: Dr Lesia Rudenko Managing Editor: Agnieszka Rosa – amarosa@wp.pl International Editorial Office: Lesia Rudenko (editor) – l.rudenko@wydawnictwo-aluna.pl Nina Radchenko (editor's assistant) – n.radchenko@wydawnictwo-aluna.pl Polish Medical Association (Polskie Towarzystwo Lekarskie): Prof. Waldemar Kostewicz – President PTL Prof. Jerzy Woy-Wojciechowski – Honorary President PTL Kris Bankiewicz San Francisco, USA Christopher Bara Hannover, Germany Krzysztof Bielecki Warsaw, Poland Zana Bumbuliene Vilnius, Lithuania Ryszarda Chazan Warsaw, Poland Stanislav Czudek Ostrava, Czech Republic Jacek Dubiel Cracow, Poland Zbigniew Gasior Katowice, Poland Andrzej Gładysz Wroclaw, Poland Nataliya Gutorova Kharkiv, Ukraine Marek Hartleb Katowice, Poland Roman Jaeschke Hamilton, Canada Andrzej Jakubowiak Chicago, USA Oleksandr Katrushov Poltava, Ukraine Peter Konturek Saalfeld, Germany Jerzy Korewicki Warsaw, Poland Jan Kotarski Lublin, Poland George Krol New York, USA Krzysztof Łabuzek Katowice, Poland Henryk Majchrzak Katowice, Poland Ewa Małecka-Tendera Katowice, Poland Stella Nowicki Memphis, USA Alfred Patyk Gottingen, Germany Palmira Petrova Yakutsk, Russia Krystyna Pierzchała Katowice, Poland Tadeusz Płusa Warsaw, Poland Waldemar Priebe Houston, USA Maria Siemionow Chicago, USA Vladyslav Smiianov Sumy, Ukraine Tomasz Szczepański Katowice, Poland Andrzej Witek Katowice, Poland Zbigniew Wszolek Jacksonville, USA Vyacheslav Zhdan Poltava, Ukraine Jan Zejda Katowice, Poland Distribution and Subscriptions: Bartosz Guterman prenumerata@wydawnictwo-aluna.pl Graphic design / production: Grzegorz Sztank www.red-studio.eu Publisher: ALUNA Publishing House ul. Przesmyckiego 29, 05-510 Konstancin – Jeziorna www.wydawnictwo-aluna.pl www.wiadomoscilekarskie.pl www.wiadlek.pl International Editorial Board – in-Chief: Marek Rudnicki Chicago, USA International Editorial Board – Members: © Aluna Wiadomości Lekarskie 2016, tom LXIX, nr 4 605 Pediatria Prof. dr hab. med. Ewa Małecka-Tendera (SUM Katowice) Dr hab. med. Tomasz Szczepański (SUM Katowice) Położnictwo i ginekologia Prof. dr hab. med. Jan Kotarski (UM Lublin) Prof. dr hab. med. Andrzej Witek (SUM Katowice) Stomatologia Prof. dr hab. Maria Kleinrok (UM Lublin) Polskie Towarzystwo Lekarskie Prof. dr hab. med. Waldemar Kostewicz (Prezes ZG PTL) Prof. dr hab. med. Jerzy Woy-Wojciechowski (Prezes Honorowy PTL) Prof. emerytowany dr hab. med. Tadeusz Petelenz (O. Katowicki PTL) Koordynator projektu Agnieszka Rosa tel. 694 778 068 amarosa@wp.pl Redakcja zagraniczna dr n. med. Lesia Rudenko l.rudenko@wydawnictwo-aluna.pl Wydawca Wydawnictwo Aluna ul. Przesmyckiego 29 05-510 Konstancin-Jeziorna www.aluna.waw.pl Prenumerata prenumerata@wydawnictwo-aluna.pl www.wiadomoscilekarskie.pl/prenumerata Opracowanie gra�czne Piotr Dobrzyński (www.poligra�a.nets.pl) Nakład do 6 tys. egz © Copyright by Aluna Publishing Wydanie czasopisma Wiadomości Lekarskie w formie papierowej jest wersją pierwotną (referencyjną). Redakcja wdraża procedurę zabezpieczającą oryginalność prac naukowych oraz przestrzega zasad recenzowania zgodnie z wytycznymi Ministerstwa Nauki i Szkolnictwa Wyższego. Czasopismo indeksowane w: PubMed/Medline, EBSCO, MNISW (11 pkt), Index Copernicus, PBL, Scopus Redaktor naczelny Prof. dr hab. med. Władysław Pierzchała (SUM Katowice) Zastępca redaktora naczelnego Prof. zw. dr hab. med. Aleksander Sieroń (SUM Katowice) Redaktor wydania prof. dr hab. Maria Majdan prof. dr hab. Mirosław Jabłoński Redaktor statystyczny dr n. med. Lesia Rudenko Rada naukowa Redaktorzy tematyczni: Chirurgia Prof. dr hab. med. Krzysztof Bielecki (CMKP Warszawa) Prof. dr hab. med. Stanislav Czudek (Onkologickié Centrum J.G. Mendla Czechy) Prof. dr hab. med. Marek Rudnicki (University of Illinois USA) Choroby wewnętrzne Prof. dr hab. med. Ryszarda Chazan, pneumonologia i alergologia (UM Warszawa) Prof. dr hab. med. Jacek Dubiel, kardiologia (CM UJ Kraków) Prof. dr hab. med. Zbigniew Gąsior, kardiologia (SUM Katowice) Prof. dr hab. med. Marek Hartleb, gastroenterologia (SUM Katowice) Prof. dr hab. med. Jerzy Korewicki, kardiologia (Instytut Kardiologii Warszawa) Dr hab. med. Krzysztof Łabuzek, farmakologia kliniczna, diabetologia (SUM Katowice) Prof. dr hab. med. Tadeusz Płusa, pneumonologia i alergologia (WIM Warszawa) Dr hab. med. Antoni Wystrychowski, nefrologia (SUM Katowice) Choroby zakaźne Prof. dr hab. med. Andrzej Gładysz (UM Wrocław) Epidemiologia Prof. dr hab. med. Jan Zejda (SUM Katowice) Neurologia i neurochirurgia Prof. dr hab. med. Henryk Majchrzak, neurochirurgia (SUM Katowice) Prof. dr hab. med. Krystyna Pierzchała, neurologia (SUM Katowice) Redaktor naczelny Prof. dr hab. med. Władysław Pierzchała (SUM Katowice) Zastępca redaktora naczelnego Prof. zw. dr hab. n. med. Aleksander Sieroń (SUM Katowice) Sekretarz redakcji Joanna Grocholska Redaktor statystyczny Ewa Guterman Rada naukowa Redaktorzy tematyczni: Chirurgia Prof. dr hab. med. Krzysztof Bielecki (CMKP Warszawa) Prof. dr hab. med. Stanislav Czudek (Onkologickié Centrum J.G. Mendla Czechy) Prof. dr hab. med. Marek Rudnicki (University of Illinois USA) Choroby wewnętrzne Prof. dr hab. med. Marek Hartleb, gastroenterologia (SUM Katowice) Prof. dr hab. med. Jacek Dubiel, kardiologia (CM UJ Kraków) Prof. dr hab. med. Zbigniew Gąsior, kardiologia (SUM Katowice) Prof. dr hab. med. Jerzy Korewicki, kardiologia (Instytut Kardiologii Warszawa) Dr hab. med. Antoni Wystrychowski, nefrologia (SUM Katowice) Prof. dr hab. med. Ryszarda Chazan, pneumonologia i alergologia (UM Warszawa) Prof. dr hab. med. Tadeusz Płusa, pneumonologia i alergologia (WIM Warszawa) Choroby zakaźne Prof. dr hab. med. Andrzej Gładysz (UM Wrocław) Epidemiologia Prof. dr hab. med. Jan Zejda (SUM Katowice) Neurologia i neurochirurgia Prof. dr hab. med. Krystyna Pierzchała, neurologia (SUM Katowice) Prof. dr hab. med. Henryk Majchrzak, neurochirurgia (SUM Katowice) Pediatria Prof. dr hab. med. Ewa Małecka-Tendera (SUM Katowice) Dr hab. med. Tomasz Szczepański (SUM Katowice) Położnictwo i ginekologia Prof. dr hab. med. Jan Kotarski (UM Lublin) Prof. dr hab. med. Andrzej Witek (SUM Katowice) Stomatologia Prof. dr hab. Maria Kleinrok (UM Lublin) Polskie Towarzystwo Lekarskie Prof. dr hab. med. Jerzy Woy-Wojciechowski (Prezes PTL) Prof. emerytowany dr hab. med. Tadeusz Petelenz (O. Katowicki PTL) Kontakt z redakcją i wydawnictwem Joanna Grocholska e-mail: j.grocholska@blue-sparks.pl Wydawca Blue Sparks Publishing Group Sp. z o.o. ul. Obornicka 15/4, 02-948 Warszawa tel. (22) 858-92-53 Zarząd: dr Anna Łuczyńska − prezes Reklama i marketing: Agnieszka Rosa tel. 662-116-020 e-mail: a.rosa@blue-sparks.pl Zamówienia na prenumeratę: e-mail: prenumerata@blue-sparks.pl lub tel. (22) 858-92-53 Projekt okładki: Dorota Cybulska Opracowanie gra� czne: Tomasz Białkowski Nakład: do 6000 egz. © Copyright by Blue-Sparks Publishing Group Wydanie czasopisma Wiadomości Lekarskie w formie papierowej jest wersją pierwotną (referencyjną). Redakcja wdraża procedurę zabezpieczającą ory- ginalność publikacji naukowych oraz przestrzega zasad recenzowania prac zgodnie z wytycznymi Ministerstwa Nauki i Szkolnictwa Wyższego. Czasopismo indeksowane w: Medline, EBSCO, MNiSW (6 pkt), Index Copernicus, PBL. Czasopismo Polskiego Towarzystwa Lekarskiego Pamięci dra Władysława Biegańskiego Wiadomości Lekarskie WL_1_2013.indb 1 27.03.2013 12:23 2022 Wiadomości Lekarskie, VOLUME LXXIV, ISSUE 9 PART 1, SEPTEMBER 2021 © Aluna Publishing FOR AUTHORS 1. 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Manuscripts not concordant with the above instructions will be returned to be corrected. 28. The editors do not return papers which have not been commissioned. 29. The editors take no responsibility for the contents of the advertisements. 2023 Wiadomości Lekarskie, VOLUME LXXIV, ISSUE 9 PART 1, SEPTEMBER 2021© Aluna Publishing CONTENTS ORIGINAL ARTICLES Aidyn G. Salmanov, Oleg M. Ishchak, Serhii А. Dobarin, Olena M. Susidko, Olena V. Mosendz, Svitlana M. Korniyenko, Oleksandr A. Voloshyn PERINATAL INFECTIONS IN UKRAINE: RESULTS OF A MULTICENTER STUDY 2025 Kateryna Hlushko, Oksana Boyarchuk, Maria Kinash, Emillia Burbela, Yana Rohalska, Lesia Dobrovolska AWARENESS OF FOLIC ACID USE AND ITS EFFECTS AMONG MEDICAL STUDENTS IN UKRAINE 2033 Iana O. Andreieva, Olha I. Riznyk, Sergii P. Myrnyi, Nikolai N. Surmylo STATE OF CUTANEOUS MICROCIRCULATION IN PATIENTS WITH OBESITY 2039 Bohdan Tataryn, Anna Kryzhanivska, Iryna Dyakiv, Alina Andriiv SURVIVAL OF PATIENTS WITH RECTAL CANCER 2044 Alina O. Ponyrko, Valentyna I. Bumeister, Serhii M. Dmytruk, Olha S. Yarmolenko, Tetiana P. Teslyk, Tetiana V. Riabenko, Iryna Shkolna STRUCTURAL CHANGES OF LONG TUBULAR BONES OF MATURE RATS UNDER THE HYPERGLYCEMIA 2052 Tetiana V. Harapko, Lesia R. Mateshuk-Vatseba MORPHOMETRIC AND ELECTRON MICROSCOPIC CHANGES OF STRUCTURAL COMPONENTS OF LYMPH NODES DURING CORRECTION OF THE ACTION OF SODIUM GLUTAMATE WITH ORLISTAT 2060 Inna I. Torianyk ULTRAMICROSCOPIC CHARACTERISTICS OF ERYTHROCYTES IN INDIVIDUALS WITH BORRELIOSIS, RHEUMATOID ARTHRITIS AND TOXOPLASMOSIS AS A MARKER, COMORBID OR CONCOMITANT PATHOLOGY FOR BABESIOSIS 2066 Kseniia Suprun, Yanina Kutasevych, Iryna Oliinyk, Oleksii Bilozorov, Iryna Mashtakova, Olga Stuliy PECULIARITIES OF TREATMENT OF PATIENTS WITH ONYCHOMYCOSIS WITH CONTRAINDICATIONS TO SYSTEMIC ANTIMYCOTICS 2070 Iryna M. Nikitina, Nataliia Ye. Horban, Kseniia V. Mykytyn, Kateryna O. Kondratiuk, Svitlana A. Smiian, Nataliia V. Kalashnyk, Nadiya V. Gerevich THE STATE OF REPRODUCTIVE HEALTH OF WOMEN WITH HYPERPROLIFERATIVE PATHOLOGY OF THE ENDOMETRIUM 2076 Olga L. Skrypa EVALUATION OF THE EFFECTIVENESS OF TREATMENT OF PATIENTS WITH TEMPOROMANDIBULAR JOINT DYSFUNCTION AFTER MANDIBULAR FRACTURES ACCORDING TO X-RAY EXAMINATION 2082 Igor P. Vakaliuk, Nataliia V. Savchuk, Roksolana V. Nesterak, Haliia B. Kulynych, Ruslana S. Hryhoryshyn INFLUENCE OF COMPLEX TREATMENT WITH MAGNESIUM AND POTASSIUM SALTS OF GLUCONIC ACID, EPLERENONE AND RIVAROXABAN ON DYNAMICS OF INDICATORS OF ISCHEMIA AND MYOCARDIAL REMODELING IN PATIENTS WITH CHRONIC HEART FAILURE AFTER MYOCARDIAL INFARCTION 2087 Oksana Ishchenko, Iryna Koshova, Inna Borysova, Dmytro Stepanskyi MICROBIOLOGICAL FEATURES OF STAPHYLOCOCCUS AUREUS ISOLATED FROM RESPIRATORY TRACT OF CHILDREN WITH CYSTIC FIBROSIS 2094 Petro A. Hasiuk, Мykhailo Ramus, Anna Vorobets, Iryna Tkachenko, Tetiana Dzetsiukh, Dmytro Kalashnikov, Svitlana Rosolovska COMPARATIVE EVALUATION OF HISTOTOXICITY INDICATORS OF METAL ALLOYS FOR THE MANUFACTURE OF METAL CERAMIC DENTAL CONSTRUCTIONS 2100 Antonina Sydorenko, Ruslan Lutsenko, Iryna Kniazkova, Nataliia Liakhovska, Olga Lutsenko PECULIARITIES OF MOTOR ACTIVITY OF RATS IN SIMULATION PHARMACOLOGICAL DEPRESSOGENIC STATES 2105 Evheniia A. Shtaniuk, Oleksandra O. Vovk, Larisa V. Krasnikova, Yuliia I. Polyvianna, Tetiana I. Kovalenko STUDY OF ANTIBACTERIAL ACTIVITY OF THE PREPARATIONS WITH DIOXIDINE AND LEVOFLOXACIN ON MAIN PURULENT-INFLAMMATORY PROCESSES PATHOGENS 2109 Natalia N. Brailko, Iryna M. Tkachenko, Victor V. Kovalenko, Anna V. Lemeshko, Alexey G. Fenko, Ruslan V. Kozak, Dmitry V. Kalashnikov INVESTIGATION OF STRESS-STRAIN STATE OF “RESTORATION & TOOTH” SYSTEM IN WEDGE-SHAPED DEFECTS BY COMPUTED MODELING METHOD 2112 Yuliya V. Sarkisova, Viktor T. Bachynskiy, Oleksandr I. Garazdiuk, Ivan V. Garazdiuk, Iana M. Teleki POSTMORTEM INTERVAL ESTIMATION BY THE METHOD OF WAVELET ANALYSIS OF STOKES-POLARIMETRIC MAPPING DATA OF HUMAN VITREOUS BODY LAYERS 2118 Volodymir K. Likhachov, Viktoriya L. Vashchenko, Olena О. Taranovska IMPACT OF PREVENTIVE THERAPY ON REGULATING MECHANISMS OF DECIDUAL MACROPHAGE POLARIZATION IN PREGNANT WOMEN WITH HIGH RISK OF PREECLAMPLSIA 2123 Zbigniew Żęgota, Joanna Goździk, Joanna Głogowska-Szeląg IMPROVED PHYSICAL FUNCTION WITH COMPLEMENTARY USE OF A DIETARY SUPPLEMENT FOR MILD KNEE OSTEOARTHRITIS: A SUBGROUP ANALYSIS 2128 2109 Wiadomości Lekarskie, VOLUME LXXIV, ISSUE 9 PART 1, SEPTEMBER 2021© Aluna Publishing INTRODUCTION Topic of nosocomial infection, particularly in surgical practice, is valid in medicine all over the world [1-3]. Nosocomial purulent-inflammatory complication is always accompanied by pathogen, degree of infection severity course, character of lesions, spreading rate, treatment ef- ficiency, etc. depend on the type of pathogen [4-6]. Growth of antibiotic-resistant strains of gram-negative and gram-positive flora reflecting general trend of micro- organisms evolution is the consequence of irrational use of antibacterial preparations, causing certain difficulties in treatment and adversely affecting clinical results. Thus the study of the spectrum of purulent-inflammatory processes pathogens, their sensitivity to antibacterial preparations and regular microbiological monitoring implementation are very important [6]. Knowledge of soft tissues anatomy and level of infection localization play an important role in the choice of adequate treatment tactics [7]. Concrete clinical situation must be analyzed accounting risk factors and epidemiologic sit- uation must be studied, which allows with a fairly high probability frequency defining potential pathogen and prescribing adequate rational empiric antibacterial therapy. THE AIM Search of most effective experimental antibacterial prepa- rations on main optional-anaerobic pathogens of nosoco- mial purulent-inflammatory surgical wounds. The aim was to study antibacterial activity of preparations containing antiseptic dioxidine and antibiotic levofloxacin on standard strains of surgical wounds pathogens S. aureus, E. coli, P. aeruginosa and find more effective ones on them. MATERIALS AND METHODS We used two solutions with dioxidine for experiment, name- ly: solution dioxidine 1.2%, solution dioxidine 1.2% with decamethaxin, Dioxisole, two water soluble ointments of dioxidine 1.2 % and levofloxacin 0.1% with decamethaxin. Antibacterial activity was studied on standard strains of S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853, received from SI «I.I.Mechnikov Institute of microbiology and immunology of the NAMS of Ukraine». Antibacterial activity of solutions and ointments was studied with the help of agar diffusion method (“well” method) according to methodic recommendations [8]. Each investigation was repeated 6 times. STUDY OF ANTIBACTERIAL ACTIVITY OF THE PREPARATIONS WITH DIOXIDINE AND LEVOFLOXACIN ON MAIN PURULENT-INFLAMMATORY PROCESSES PATHOGENS DOI: 10.36740/WLek202109115 Evheniia A. Shtaniuk, Oleksandra O. Vovk, Larisa V. Krasnikova, Yuliia I. Polyvianna, Tetiana I. Kovalenko KHARKIV NATIONAL MEDICAL UNIVERSITY, KHARKIV, UKRAINE ABSTRACT The aim: Study of antibacterial activity of the preparations, containing antiseptic dioxidine and antibiotic levofloxacin in vitro on standard strains of main optional-anaerobic pathogens of purulent-inflammatory processes of surgical wounds S. aureus, E. coli, P. aeruginosa and definition of more effective ones on them. Materials and methods: Solutions of dioxidine 1.2 %, dioxidine 1.2% with decamethaxin, Dioxisole, water soluble ointment with dioxidine 1.2% and levofloxacin 0.1% with decamethaxin were used in experiment. Antibacterial activity was studied on standard strains of S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Distinguishing and identification of pure cultures of bacteria was done according to generally accepted microbiological methods. Determination of purulent-inflammatory processes pathogens sensitivity was done by disco-diffuse method on Mueller-Hinton medium. Antibacterial activity of solutions and ointments was studied with the help of agar diffusion method (“well” method) according to methodic recommendations. Each investigation was repeated 6 times. Method of variation statistics was used for the research results analysis. Results: All antibacterial preparations under study are effective and highly effective on S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Solution with 1.2 % dioxidine with decamethaxin and ointment with 0.1 % levofloxacin and decamethaxin have larger growth retardation zones towards S. aureus and P. aeruginosa. E. coli strains are more sensitive to the solution of Dioxisole and ointment with 1.2 % dioxidine. Conclusions: All strains are sensitive, most of them are highly sensitive, up to 5 antibacterial preparations under study in vitro. KEY WORDS: dioxidine, levofloxacin, S. aureus, E. coli,P. aeruginosa Wiad Lek. 2021;74(9 p.I):2109-2111 ORIGINAL ARTICLE Evheniia A. Shtaniuk et al. 2110 Method of variation statistics was used for the research results analysis. Reliability of the received results were determined by Student criterion (at р < 0.05) [9]. RESULTS Antibacterial preparations results study in vitro on standard strains of purulent-inflammatory processes pathogens are given in the Table I. We can conclude that all strains are sensitive; most of them are highly sensitive, up to 5 antibacterial preparations under study. Retardation zones are from 23.32 to 32.77 mm. Solution with 1.2% dioxidine has high growth retardation zones towards S. aureus and P. aeruginosa (24.68 and 23.32 mm) and the largest growth retardation zone on standard strain of E. coli АТСС 25922 and comprises 27.32 mm. As for solutions of 1.2 % dioxidine with decamethaxin and Dioxisole, they were effective on all standard strains (growth retardation zones were from 27.07 to 34.22 and from 26.63 to 31.93 mm, consequently). Experimental ointment with 1.2 % dioxidine is highly effective on E. coli АТСС 25922, P. aeruginosa АТСС 27853 (31.58 and 30.02 mm, consequently) and effective on S. aureus АТСС 25923 (24.42 mm). Ointment with 0.1% levofloxacin and decamethaxin has high growth retarda- tion zones of all standard strains from 26.1 to 32.77 mm. DISCUSSION We studied research results of other scientists. The bacte- ricidal effect on staphylococci is exerted by whole dioxidin and dioxidin in a 1: 1 dilution. The zone of inhibition of the growth of microorganisms was more than 10 mm. Dioxidine in a dilution of 1: 5 showed a bacteriostatic effect. In this case, the zone of inhibition of the growth of micro- organisms was up to 10 mm. Also dioxidine in a dilution of 1: 1 and 1: 5 had a stable bactericidal effect on E. coli and P. aeuriginisa [10]. It is known from other scientific sources that dioxidine is active in therapeutic concentrations against abso- lutely the majority of the studied strains, including the hospital microorganisms that are polyvalent resistant to antibacterial drugs [11]. Ointments on a water-soluble basis, containing of levoflaxacin (Levomecol) or 5% of dioxidine are effective for treatment of wounds in the first phase of the wound process. It is recommended to use 5% dioxidine ointment for treatment of wounds, which couses by gram-negative microflora, in partic- ular, Pseudomonas aeruginosa, Also, this ointment is effective in relation to anaerobic microflora in combination with aerobic one. In the treatment of the second phase of the wound process at the stage of maturation of granulations, you can use a 1% solution of dioxidine and ointments on a water-soluble basis [12]. In our studies, all the studied drugs with dioxidine and levofloxacin showed bactericidal action in relation to all standard strains of S. aureus, E. coli and P. aeruginosa. E. coli was most sensitive on the solution of Dioxisole and oint- ment with 1.2 % dioxidine. Solution with 1.2 % dioxidine with decamethaxin and ointment with 0.1 % levofloxacin and decamethaxin had the largest growth retardation zones on S. aureus and P. aeruginosa. CONCLUSIONS 1. All antibacterial preparations under study are effective and highly effective on S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. 2. Solution with 1.2 % dioxidine with decamethaxin and ointment with 0.1 % levofloxacin and decamethaxin have the largest growth retardation zones on S. aureus АТСС 25923 (27.07 and 29.75 mm) and P. aeruginosa АТСС 27853 (34.22 and 32.77 mm). 3. E. coli АТСС 25922 is most sensitive in vitro on the solution of Dioxisole and ointment with 1.2 % dioxidine (31.93 and 31.58 mm, consequently). 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Antibacterial activity of the preparations in the way of solutions and ointments on standard strains of S. aureus, E. coli and P. aeruginosa Bacteria strain test Growth retardation zones diameter, mm (M ± m) at n = 6 Sol. D* Sol. D+D** Sol. Dioxisole Ointment 1.2 % dioxidine Ointment L+D* S. aureus АТСС 25923 24.68±0.74 27.07±0.58 26.63±0.42 24.42±0.38 29.75±0.80 E. coli АТСС 25922 27.32±0.92 29.72±0.88 31.93±0.56 31.58±0.48 26.1±0.52 P. aeruginosa АТСС 27853 23.32±0.88 34.22±0.50 30.02±0.52 30.02±0.71 32.77±0.54 Note: Sol. D* – 1.2 % dioxidine; Sol. D+D** – 1.2 % dioxidine + decamethaxin; ointment L+D* – 0.1 % levofloxacin + decamethaxin. STUDY OF ANTIBACTERIAL ACTIVITY OF THE PREPARATIONS WITH DIOXIDINE AND LEVOFLOXACIN... 2111 5. Formulary of drugs registered in Ukraine as of 23.04.2018. https:// medhub.info/31246dcb (in Ukrainian). 6. Yakimenko Е.А., Tbileli V.V., Zakatova L.V. et al. Approaches to choosing starting antibacterial therapy. 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Choice of topical preparations. Modern principles of treatment of purulent wounds, St. Petersburg: Onpi-Press; 2017, 20-24 p. (in Russian). 0118U000930 «Improvement of diagnostics and treatment methods of purulent-inflammatory diseases caused by op- portunistic microorganisms». ORCID and contributionship: Evheniia A. Shtaniuk: 0000-0002-1077-2819 A,B Oleksandra O. Vovk: 0000-0002-0649-3163 C Larisa V. Krasnikova: 0000-0001-6675-4495 D Yuliia I. Polyvianna: 0000-0001-8092-8164 E Tetiana I. Kovalenko: 0000-0002-5687-2583 F Conflict of interest: The Authors declare no conflict of interest. CORRESPONDING AUTHOR Evheniia A. Shtaniuk Kharkiv National Medical University 4 Nauki avenue, 61022 Kharkiv, Ukraine tel: +380509473130 e-mail: evshtanyuk@gmail.com Received: 17.09.2020 Accepted: 02.08.2021 A – Work concept and design, B – Data collection and analysis, C – Responsibility for statistical analysis, D – Writing the article, E – Critical review, F – Final approval of the article