Кафедра фізіології
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Item A study of enterocyte membranes during activation of apoptotic processes in chronic carrageenan-induced gastroenterocolitis(2018-08) Tkachenko, А.; Marakushyn, Dmytro; Kalashnyk, Yulia; Onishchenko, A.; Gorbach, Tetiana; Nakonechna, O.; Posokhov, Y.; Tsygankov, A.; Korniyenko, Ye.Aim To investigate the lipid membranes of rat enterocytes in chronic carrageenan-induced gastroenterocolitis accompanied by the activation of apoptotic processes. Methods Steady-state fluorescence spectroscopy: a study by fluorescent probes - by ortho-hydroxy derivatives of 2,5-diaryl-1,3- oxazole. Activity of apoptosis signal-regulating kinase 1 and poly (ADP-ribose) polym erase in small intestinal homogenates, blood serum levels of matrix metalloproteinase-2 and caspase-3 and the level of DNA fragmentation in small intestinal homogenates were determined. Results Biochemical analysis revealed that an activation of apoptotic processes occurred in the intestinal epithelium of rats during chronic carrageenan-induced gastroenterocolitis. The fluorescence probes showed that activation of apoptotic processes in carrageenan-induced gastroenterocolitis was accompanied by changes in polar regions of rat enterocyte membranes, while no changes were revealed in more hydrophobic regions of the membranes. Conclusion The increase in hydration of membranes was attributed to the activation of the apoptosis of enterocytes. It has been shown that a fluorescent probe (2-(2'-hydroxyphenyl)-5-phenyl-l,3-oxa- zole) can be used for the detection of apoptosis of enterocytes.Item A study of erythrocyte membranes in carrageenaninduced gastroenterocolitis by method of fluorescent probes(2018-04) Tkachenko, A.; Marakushyn, Dmytro; Rezunenko, Y.; Onishchenko, A.; Nakonechna, O.; Posokhov, Y.Objective. The aim of our research was to investigate erythrocyte membranes of rats with carrageenan-induced gastroenterocolitis using fluorescent probes (ortho-hydroxy derivatives of 2,5-diaryloxazole).Materials and methods. Fluorescence of O1O (2 - (2’-OH-phenyl)5-phenyl-1,3-oxazole) and PH7 (2-(2’-OH-phenyl)-phenanthro[9,10]-1,3-oxazole) probes in suspensions of erythrocytes of rats with chronic carrageenan-induced gastroenterocolitis was determined and compared to the control group consisted of intact animals.Results. It has been found that the hydration of erythrocyte membranes increases in rats with carrageenan-induced gastroenterocolitis. The disease is associated with the changes in the polar regions of the membranes, while no changes in the hydrophobic areas of the erythrocyte membranes are observed. Conclusion. The detected changes allow us to make a conclusion that the fluidity of the erythrocyte membranes of the rats with chronic carrageenan-induced gastroenterocolitis increases.Item A study of influence of working at the computer on some indexes of the physical health state of students(2018-09) Gordienko, P.; Pandikidis, N.; Shenher, S.The purpose of the work is to study the level of physical health, ability to work dynamics during the week, prevalence of Internet addiction and the impact of working at a computer on the students’ health state.Item Acute myeloid leukemia(Харківський Національний Медичний Університет, 2017-05-16) Bajbouj, Omar; Alsharif, Mahmoud; Hloba, Nataliia; Karmazina, IrynaAcute myeloid leukemia (AML) that is also known as non-lymphocytic anemia. Based on 2009–2013 cases and deaths analysis, the number of new cases of acute myeloid leukemia was 4.1 per 100,000 men and women per year and the number of deaths was 2.8 per 100,000 men and women per year. In 2016 AML constituted 19,950 from estimated new cases and 1.2 % of all new cancer cases with 10,430 of estimated deaths and 1.8 % of all cancer deaths. According to data review of 2006–2012, percent of patients surviving 5 years is 26.6 %. Based on 2011–2013 data, approximately 0.5 percent of men and women will be diagnosed with acute myeloid leukemia at some point during their lifetime, thus AML is the most common leukemia in adults, that proves the importance of its research.Item Adaptability of cardiorespiratory system in normotensive and hypotensive female students with different impact of the autonomic nervous system subdivisions(2019-05-15) Goncharova, Аlina; Pavlov, S.; Pavlova, O.; Razumovskiy, A.; Kaur, A.It was carried out the analysis of the body adaptability in the situation of training loads stress in students with normotension and hypotension and predominance of different types of autonomic nervous system regulatory influence on the cardiorespiratory system. In 103 second-year female students of KNMU systolic, diastolic blood pressure, pulse pressure, heart rate were measured, the Kerdo index and the Robinson index were determined. Functional breathing tests with breathing delay on the inhalation (Stange) and on the exhalation (Genchi) were conducted. They are the indicators of the cardiorespiratory system functional state and sensitivity to a decrease of oxygen saturation in arterial blood (hypoxemia) and carbon dioxide increase in blood (hypercapnia). Psycho-emotional stress in second-year female students causes a deficiency in the body adaptation reserves. Formation of adaptating to learning stress is carried out at the limit of the functional capabilities of the body and may lead to a breakdown of adaptationItem Adaptation index and functional state of central nervous system in medical students during the period of intensive learning activity(2019-05-15) Vaschuk, Мykola; Sokol, Olena; Khorshunova, Anastasiia; Chernysh, Hanna; Yacenko, AlinaIn research, an assessment has been conducted of the adaptation potential indicators of medical students with different individual-typological features of higher integrative processes with the determination of the functional state of the CNS by the following quantitative criteria: functional level of the system, stability of the reaction and level of functional capabilities that were determined using simple visual motor reaction. The property of functional mobility was determined by the magnitude of the maximum speed for an unmistakable differentiation of positive and inhibitory stimuli. The adaptation score was calculated using the L.H. Harkavy with distribution by type of adaptive response of female students: Group 1 (2%) with stress response; Group 2 (17%) with a workout reaction; Group 3 (31%) with a calm activation reaction; Group 4 (47%) with an increased activation reaction and Group 5 (3%) with a reactivation reaction. During the initial stages of stress, an increase in excitation processes was observed. During the training reaction, a low level of immune system reactivity is reflected in the severity of inhibitory processes. With calm activation, inhibition processes intensified in the physiological range. With increased activation, the processes of excitation rather predominated over the processes of inhibition. During the reactivation reaction, excessive excitation processes prevailed.Item Adipokines and interleukin-17 are the link of regulatory mechanism in kidney(2018-09-28) Goncharova, АlinaAdipokines and interleukins (IL) are involved in regulation of renal function but their role in the mechanisms is not clear yet. If the effects of such adipokine as adiponectin in kidney disorders are better defined [1], the effects of visfatin and IL17 are not so well studied.Item Aggressive parenting: social, medical and legal aspects(2024-01) Isaieva, Inna; Isaiev, Arsen; Korobtsova, Nataliia; Nadon, Victoriia; Puchkovska, IrynaAggressive parenting is considered as style using threats or different kinds of punishments such as psychological or physical to keep a child under control or pressure to obey. However, the standardized definition of aggressive parenting doesn’t exist and most of interpretations are focused on physical aggression which refers to actions with intention of causing physical pain or injury.Item Analysis of heart rate variability as a potential marker of shock factors(2021-10-28) Pandikidis, N.; Maslova, Natalia; Maslova, YuliiaChanges in the functional state of the autonomic nervous system are accompanied by various functional states of the heart itself, as well as cardiac and non-cardiac diseases. Was conducted an analysis of the literature of recent years, the importance of analyzing heart rate variability in diagnosing various lesions of the central nervous system, as well as predicting the survival of patients.Item Anthropological aspects of creative thinking of a person with type-forming premises on the psychophysiological background(2018-11-08) Vashchuk, N.; Tishchenko, M.Актуальностью темы является разработка компетентностных подходов к определению общих способностей и творческого мышления [1,5,6]. Системный подход к процессу творчества трехаспектный, интегрирующие его составляющие: предметная, социальная и личностная [2,4]. Поэтому становление интеллекта необходимо рассматривать как сложный многомерный процесс. В www.iscience.me 123 последнее время в психофизиологии творчества решающую роль приобретает социокультурная детерминация, которая рассматривает развитие знаний о человеке как результат культурно-эволюционного развития [3]. Эволюционная физиология стала определять стиль мышления в психологии. На разных уровнях эволюции увеличивается вариативность психических способностей индивидов, входящих в расширяющиеся системы, а также разновидность проявлений человека в биогенезе, антропогенезе, социогенезе и персоногенезе [7,8]. На современном этапе особую значимость приобретают две исследовательские ориентации. Психофизиология находит новые связи между творческой активностью мозга[1,9].Item Application of cardioplegic solution in clinical practice(Харківський Національний Медичний Університет, 2007-05-16) Bibhu, Charan Nayak; Hloba, Nataliia; Isaieva, InnaItem Application of simulation technologies in teaching of theoretical disciplines in medical university(2017-05-18) Chernobay, Larysa; Hloba, Nataliia; Isaieva, Inna; Karmazina, IrynaModern higher medical education undergoes the process of modernization aimed at creation of necessary conditions for professional training of specialists with high level of competence, creative thinking and ability to make valid responsible decisions in process of their professional activity. Reaching of that aim requires changes of traditional ways of educational and pedagogical interaction between teachers and students and including of modern innovation technologies in training process. Taking into account the specificity of professional activity of future doctors, simulation technologies become one of the most important parts of higher medical education. Application of simulation technologies aimed at imitation of real process of doctor’s work allows teachers to form in students during training process all the theoretical knowledge, practical skills and personal traits necessary for their future professional activity.Item Architecture of a functional system of the sagittal balance maintaining(Polish Physiological Society, 2021-09-15) Goncharova, AlinaTo understand the mechanisms of maintaining the sagittal balance within physiological borders, it is important to consider it within the p.K. anokhin’s concept of functional system. Consideration of the structures involved in maintaining the sagittal balance from this point of view will allow us to determine the possible causes of its displacement and methods of its correction.Item Aspects of diagnostic criteria in women with luteal insufficiency(2018-10-06) Tishchenko, A.; Tishchenko, M.Недостаточность лютеиновой фазы (НЛФ) - является гормональным нарушением, все многообразие которого реализуется на уровне эндометрия. Недостаточная гормонально зависимая структурная и функциональная перестройка эндометрия может приводить к отсутствию или дефектам имплантации. Одним из основных секреторных белков эндометрия является альфа-2-микроглобулин фертильности (АМГФ).Item Assessment of the time course of the state of extraocular muscles in printed load with different design parameters(2018-09-30) Maslova, NataliaThe study deals with evaluation of the time course of the state of extraocular muscles of the visual system in 199 pupils of three age groups in printed load, estimated from the ratio of the interference pattern parameters and the symmetry factor introduced by the author. Adaptation of the visual system to its own state (structural and functional organization) was found to occur in many cases due to asymmetric functioning of extraocular muscles. In long work at a close distance this initial (adaptivecompensatory) asymmetry, providing binocular vision, converges with asymmetry caused by visual stress and fatigue. Thus, the study identified three main ways of adapting to work at close distance. KeywordsItem Autonomic supply of physical activity in young people with arterial hypotension(2020-02) Marakushyn, Dmytro; Chernobay, Larysa; Isaieva, Inna; Karmazina, Iryna; Goncharova, Аlina; Shenger, Svitlana; Bulynina, Oksana; Sokol, OlenaTotally 128 young people aged 17–21 were examined including 50 individuals with normal values of blood pressure and 78 people with arterial hypotension. Young people with arterial hypotension were ranged according to the level of arteral pressure: first group included 39 persons (moderate arterial hypotension) where the level of mean arterial pressure was determined as 80–75 mm Hg; the second group (expressed arterial hypotension) included 39 people with level of mean arterial pressure 74.9–70 mm Hg followed by an analysis of the nature of the adaptive capacity of cardiovascular system to exercise and in the recovery period.Item Biotechnology production of medium for cultivation and lyophilization of lactic acid bacteria(Alex Matsyura Publishing, 2018) Gujvinska, S.; Paliy, A.; Dunaieva, Olha; Paliy, A.; Berezhna, N.The aim of our work was to develop a medium for the cultivation of lactic acid bacteria and to select the optimal protective mediums for the freeze-drying of lactic acid bacteria. The objects of research were cultures of microorganisms Lactobacillus plantarum 7 and Lactobacillus casei 27 isolated, selected and stored in the National Scientific Center Institute of Experimental and Clinical Veterinary Medicine (NSC "IECVM", Kharkiv). Cultivation of lactobacilli was carried out on medium MRS and Blaurock for 24-72 hours at the temperature of 37 °C. To develop nutrient media for the cultivation of lactobacilli, the selected components in the main composition of the medium: lactose, sodium phosphate disubstituted, sodium citrate. As components of the protective medium, solutions of sucrose, gelatinase, sodium acetate, and aerosol® were used. Because of the studies, the optimal composition of the nutrient medium was determined (lactose 1.2 %, sodium phosphate disubstituted 0.3 %, and sodium citrate 0.6 %) for the cultivation of lactic acid bacteria on which bacteria grow and actively accumulate a significant number of viable cells from 7.1 to 8.2 × 106 CFU/cm3. In the various cultivation regimes of lactobacilli, pH 7.0 is optimal, and the temperature of the inoculum incubation is 37±0,5 °C. An alternative protective medium is added with the addition of 10 % sucrose, 1.0 % gelatinase and 3.0 % aerosil, which ensures the viability of viable cells after lyophilization of strains of L. plantarum 7 and L. casei 27, respectively, 96 % and 97 %. A culture medium was proposed (lactose 1.2 %, sodium phosphate disubstituted 0.3 %, sodium citrate 0.6 %) for cultivation of lactobacilli on which bacteria grow and actively accumulate a significant number of viable cells (7.1-8.2) × 106 CFU/cm3). Under different temperature regimes and pH of the medium, the best is that it has a pH of 7.0 and the incubation of the inoculum passes at a temperature of 37 ± 0.5 °C. The maximum preservation of viable cells after lyophilization of strains L. plantarum 7 and L. casei 27, respectively, 96 % and 97 % were observed using a protective medium supplemented with 10 % sucrose, 1.0 % gelatose and 3.0 % aerosil. The addition of protective media according to the given formulation with the freeze-drying of lactic acid bacteria and the proposed drying regime have technological advantages and ensure the preservation of the basic biological properties of microorganisms.Item Blood pressure levels in trained and untrained foreign students(Харківський національний медичний університет, 2017-05-16) Sridharan, Sabareesh; Kalaivani, Baskar; Kandeeswari, Rajasaimani; Hloba, NataliiaItem Bone remodeling regulation in the model of combined unloading with chronic inflammation in rats(2021-04-06) Goncharova, AlinaCombined effect of mechanical unloading with induced chronic inflammation results in dysregulation of the mechanisms of bone homeostasis control.Item Brain integrated activity in medical students under conditions of adaptation to psychoemotional stress(2019-03) Marakushyn, Dmytro; Chernobay, Larysa; Vasylieva, O.; Morozov, O.; Korobchanskyi, PavloAccording to the results of the study the development of adaptation to psychoemotional stress, which is considered as an emotional stress in medical students during the first three years of study, has a dynamic and multifactorial character. The degree of adaptation processes’ manifestation is analyzed and it is shown that it correlates with the intellectual capacity, which is based on the features of the brain integrative activity.