Browsing by Subject "alzheimer’s disease"

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    Aducanumab and its uses in treatment of alzheimers disease
    (2017) Aleksandrova, Alina; Mukherjee, P.
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    Behavioral reactions and cognitive functions in rats with vascular model of alzheimer's type dementia at different stages of disease before and after stem cell correction
    (Kharkiv National Medical University, 2020) Lukyanova, Y.; Nikolaeva, O.; Pavlova, Olena; Vasylyeva, Irina; Naglov, O.; Shchegelskaya, Elena; Omelchenko, Elena
    The recent investigations offer to conduct the study of Alzheimer disease (AD) mechanisms using diverse experimental models. However, behavioral and cognitive impairment in rats at the different stages of vascular model of dementia of Alzheimer's type developed by us has not been investigated. Subjects and methods. The experiment was performed on 32 male WAG rats weighing 180–250 g which were divided into 4 groups. Rats from group 1 and 3 were injected aqueous solution of sodium nitrite at a dose of 50 mg/kg of body mass intraperitoneally during 14 and 28 days respectively. Groups 2 and 4 received 500,000 mesenchymal stem cells in suspension intravenously against a background of experimental nitrite-induced AD. To estimate the behavioral reactions and cognitive functions the Open Field Test (OFT) and Passive Avoidance test (PAT) were used. Results. In all experimental groups in most cases significant decrease in vertical and horizontal activity (p <0.05) and an increase in the number of defecation in the OFT were found. Rats from group 3 had the drop in locomotor, research and orientation activity. In the OFT and PAT in groups 2, 4 improvement in research activity and significant cognitive functions recovery was observed (p=0.012) after stem cell correction. Conclusion. Progression of the protective inhibition and cognitive impairment was found during the experiment. Stem cell administration had positive effects on brain function recovery.
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    Donepezil hydrochloride in the treatment of senile Alzheimer's disease, early stage
    (Kharkiv National Medical University, 2014-05-15) Berezhna, A.; Reznichenko, E.; Pavlova, O.
    We observed five patients aged 75 to 82 years, two women and three men. Memory loss, impaired concentration, mild depression was observed in all patients. Based on clinical and paraclinical methods was diagnosed Senile Alzheimer's disease early stage. In the combined therapy was appointed donepezil hydrochloride ("Alzepil") at a dose of 5 mg at bedtime for 3 months.
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    Dynamics of indicators of the endothelium morphofunctional state of the brain microcirculatory bed vessels in rats with nitrite-induced alzheimer's type dementia on the background of mesenchymal stem cell administration
    (2021) Zorenko, Yevgeniya; Gubina-Vakulyck, Galina; Pavlova, Olena; Gorbach, Tetiana; Shchegelskaya, Elena; Omelchenko, Elena
    Objective. The aim of this study was to assess the vascular endothelium morphofunctional state of the brain microcirculatory bed in rats with nitrite-induced Alzheimer's type dementia on the background of stem cells administration. Methods. 14 days after the experiment’s end, the endothelin-1, VEGF-A, eNOS, von Willebrand factor were determined in blood serum by the enzyme immunoassay and photometric methods in rats with a model of nitrite-induced dementia (14 and 28 days of sodium nitrite intraperitoneal introduction) with and without mesenchymal stem cells (MSCs) administration. The brain slices were stained according to the Einarson’s method and immunohistochemically by staging the reaction with antibodies to VEGF. Results. With an increase in the sodium nitrite administration period, the degree of damage of brain capillaries and neurons increased, dystrophy of “surviving” neurons developed and ability to produce VEGF decreased. After 14 days of “regeneration period” in groups without MSCs administration, further stimulation of VEGF production by endotheliocytes, cortex and hippocampus neurons of varying degrees was observed. In groups where stem cells were introduced, the number of capillaries increased, with endothelial hyperplasia in some cases. Conclusion. In animals with nitrite-induced dementia, dose-dependent damage to the endothelium of the capillary bed is noted. From the first day damage the vascular regeneration can be proved by VEGF expression. The stem cells administration more effectively stimulates capillary regeneration, as evidenced by a noticeable increase of the number of brain capillaries.
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    Dynamics of indicators of the endothelium morphofunctional state of the brain microcirculatory bed vessels in rats with nitrite-induced Alzheimer's type dementia on the background of mesenchymal stem cell administration or without it
    (2021) Zorenko, Yevgeniya; Gubina-Vakulyck, Galina; Pavlova, Olena; Gorbach, Tetyana; Shchegelskaya, Elena; Omelchenko, Elena
    The aim of this study was to assess the vascular endothelium morphofunctional state of the brain microcirculatory bed in rats with nitrite-induced Alzheimer's type dementia on the background of stem cells administration or without it. In animals with nitrite-induced dementia, dose-dependent damage to the endothelium of the capillary bed is noted. From the first day damage the vascular regeneration can be proved by VEGF expression. The stem cells administration more effectively stimulates capillary regeneration, as evidenced by a noticeable increase of the brain capillaries number.
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    Levels of proinflammatory cytokines IL-17 and IL-23 in patients with Alzheimer’s disease, mild cognitive impairment and vascular dementia
    (2021-01) Dubenko, Olga; Chyniak, Olha; Potapov, Olexander
    The aim: To research differences of interleukin (IL)-17 and IL-23 serum levels in patients with Alzheimer’s disease, vascular dementia and mild cognitive impairment. Material and methods: Serum levels of IL-17 and IL-23 were measure by ELISA for 15 patients with Alzheimer’s disease, 14 with vascular dementia, 30 with mild cognitive impairment and 30 control individuals without cognitive impairment. Results: Serum concentrations of IL-17 were significantly higher in Alzheimer’s disease patients (P=0.0023) than control, in vascular dementia no significant differences (P=0.4154). Level of IL-23 was significantly higher than control in Alzheimer’s disease patients (P=0.0170) and vascular dementia (P=0.0002), but in Alzheimer’s disease it was in 12.5 time higher. In total mild cognitive impairment patients no significant differences in interleukin concentration with control, but significant differences observed for amnestic form in IL-17 (P=0.0436) and IL-23 (P=0.0019). Conclusions: IL-17 and IL-23 level significant higher in Alzheimer’s disease patients compared with control and vascular dementia. From mild cognitive impairment levels of detectable interleukins was higher in amnestic form that may be early marker of progression in Alzheimer’s disease.
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    The phenomenon of self-organization of biological systems in the context of medical research
    (2017-05-10) Morozova, Okcana; Knigavko, Volodymyr
    Концепция динамической самоорганизации позволяет нам построить математическую модель для сложной биологической системы и позволяет понять принципы поведения изучаемой системы. Многие исследования показали, что динамические свойства клеточных структур согласуются с ролью самоорганизации в их формировании и функционировании. Динамические и статистические методы, используемые для изучения электрокардиограммы (ЭКГ), показывают, что сердечный ритм, скорость - нерегулярны, что указывает на то, что система далека от термодинамического равновесия, что позволяет используя нелинейную динамику модели, применяемую в электрофизиологии и кардиологии, описать использование многомасштабных систем.
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    Вehavioral reactions and cognitive functions in rats with vascular model of alzheimer's type dementia at the different stages of disease before and after stem cell correction
    (2020) Lukyanova, Yevgeniya; Nikolaeva, Olga; Pavlova, Olena; Vasyleva, Iryna; Naglov, Olexander; Shchegelskaya, Elena; Omelchenko, Elena
    In all experimental groups in most cases it was found the significant decrease in vertical and horizontal activity (p <0,05) and an increase in the number of defecation in the OFT. Rats from group 3 had the drop in locomotor, research and orientation activity. In the OFT and PAT in groups 2,4 it was observed an improvement in research activity and significant cognitive functions recovery (p=0,012) after stem cell correction. It was found the progression of the protective inhibition and cognitive impairment during experiment. The stem cells introduction had positive effects on brain function recovery