Browsing by Author "Bondarenko, Andriy"
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Item Anaplasmosis: experimental immunodeficient state model(2019) Bondarenko, Andriy; Pokhil, Sergey; Lytvynenko, Marianna; Bocharova, Tetiana; Gargin, VitaliyIntroduction: The recently described anaplasmosis infection is widespread but concerns to the insufficiently known group of diseases. The aim of our research is the development of uniform biological model for reproducing of artificial immunodeficient state by experimental anaplasmosis. Materials and methods: Algorithm of experimental anaplasmosis reproducing, consisted of such consecutive stages: 1) artificial forming of the immunodeficient state at nonlinear white mise (Mus musculus L.); 2) preparation of the tested biological material samples; 3) inoculation by prepared samples of the laboratory animals with the artificially formed immunodeficient state; 4) sampling from the dead or slaughtered (by the method of chloroformed anesthesia) experimental animals of sectional material (organs and targets tissues); 5) verification of aetiology by express detection of causative agents by the method of PCR in the selected samples of sectional material. Results: Biological model of experimental anaplasmosis have been created suitable for realization of both diagnostic and epidemiological, epizootic, ecobiological and other researches of different origin biological material samples, including samples of solid and liquid consistency material. Formed model realised in premature death of experimental animals in 17.4 % cases; resulted in an onset of disease clinical signs without death during the term of supervision in 43.8 % cases; coursed in the absence of the expressed symptoms of infection in 31.3 % cases. Conclusions: Developed biological model of experimental anaplasmosis consists in that as laboratory animals with the increased sensitiveness to the infection and accumulation of causative agent are used white nonlinear mice with the artificially formed immunodeficient state.Item Applied problems of information systems operation(2023) Dotsenko, Nataliia; Chumachenko, Igor; Bondarenko, Andriy; Chumachenko, DmytroItem Experimental-Biological Model of Bartonellosis(2017) Bondarenko, Andriy; Katsapov, Dmytro; Pochyl, Sergiy; Kozko, VolodymyrBartonella are known to be important causes of zooanthroponotic diseases. The range of human infection varies from mild lymphadenopathy and asymptomatic bacteremia to life-threatening systemic disease in immunocompromised patients. Microbiological improvements in isolation methods and PCR amplification of organism-specific DNA sequences have resulted in a dramatic increase in reports describing human patients with bartonellosis. Nevertheless, clearly and successful isolation of Bartonella spp. from bacteremic animals and human patients remains an ongoing challenge. Technology of experimental bartonellosis due to intraperitoneal introduction of biological material samples containing causative agents to laboratory animals is presented in the article. White nonlinear mice with the artificially cyclophosphamide formed immunodeficient state may be used as an experimental model for further investigation of the biological alterations responsible for angiomatosis. On the other hand, we believe that this new method will enhance the diagnostic sensitivity and specificity needed to achieve a diagnosis of bartonellosis.Item Immunization of adults(2018) Bondarenko, AndriyThe nature and function of passive and active immunization, the role of humoral and cellular immunity in vaccination, the types of vaccines currently available and the pros and cons of their use is given in the lecture. Recommended immunization schedule for adults is highlighted.Item Implementation and analysis of Babesia immunoassay testing(2020) Pokhil, Sergiy; Bondarenko, Andriy; Bocharova, Tetiana; Lepilina, Kseniia; Lytvynenko, Marianna; Gargin, VitaliyLifelong withdrawal from the donor population of those who have been diagnosed with babesiosis must be used for transmission prevention. The aim of the study was a detection of Babesia antibodies level with the usage of experimental Babesia divergens whole-cell slide antigen and commercial B. microti immunofluorescence assay substrate slide (Fuller Laboratories, USA). Methods. Experimental B. divergens whole-cell slide antigen in addition to commercial B. microti IFA substrate slide was used to create a diagnostic kit for serum Babesia antibodies level detecting, as well as for a babesiosis serodiagnosis clinical trial of different origins blood samples (patients with Lyme disease, rheumatoid arthritis and toxoplasmosis; human blood donors; cattle). Results. Antibodies to B. divergens (5.4%) and B. microti (2.3%) were detected with higher (p <0.05) frequency at Lyme disease patients (16.7%) than at blood donors (1.7%). Diagnostically significant IgG titres (= 1:128) were found in 13.3% of blood samples from Lyme disease patients and 1.7% from blood donors. Specific IgM were also found in 13.3% blood samples from Lyme disease patients. Among blood samples from Lyme disease patients, in which diagnostically significant titres of Babesia antibodies were detected (16.7%), 60% of them were represented by IgG and IgM (rA= 0.63), and in 40% only one of them reached diagnostically significant titre. Conclusions. Advantages of babesiosis IFA diagnostics are combined with its significant disadvantages (principle of evaluation, low sensitivity in the initial period of the disease, probability of false positives, absence of validated test systems and research protocols for B. divergens and B. divergens-like species).Item Implementation and analysis of Babesia immunoassay testing(Polish Medical Journal, 2020) Pokhil, Sergiy; Bondarenko, Andriy; Bocharova, Tetiana; Lepilina, Kseniia; Lytvynenko, Marianna; Gargin, VitaliyItem Infectious diseases test tasks for preparation to the state licensed examination "Krok-2. Medicine" (booklet 2018) : methodical recommendations for students of V–VІ years of higher medical education(2019) Kozko, Volodymyr; Bondarenko, Andriy; Katsapov, Dmytro; Merkulova, Nina; Gradil, Grigory; Yurko, Kateryna; Mohylenets, Olena; Sokhan, Anton; Solomennik, Ganna; Tkachenko, Vitaliytest tasks on infectious diseases for preparation to the state licensed examination "Krok-2. Medicine" (booklet 2018) are given in the methodical recommendations with explanation of correct answer for students of V and VІ years of education of medical speciality.Item Introduction to Infectious Diseases(2017-10-24) Bondarenko, AndriyThe review is focused on general questions of the etiology, epidemiology, pathology, diagnostic techniques, treatment and prevention of infectious diseases.Item Workbook for practical studies on the course of infectious diseases for the foreign faculty students of the V year of education(2017) Kozko, Volodymyr; Bondarenko, Andriy; Katsapov, Dmytro; Iurko, Kateryna; Mohylenets, Olena; Gradil, Grigory; Sokhan, AntonWorkbook for practical studies on the course of infectious diseases for the foreign faculty students of the V year of educationItem Workbook for practical studies on the course of infectious diseases for the foreign faculty students of the V year of education(2018) Kozko, Volodymyr; Bondarenko, Andriy; Katsapov, Dmytro; Yurko, Katerina; Gradil, Grigory; Mohylenets, Olena; Sokhan, AntonWorkbook for practical studies on the course of infectious diseases for the foreign faculty students of the V year of education contains educational plan of practical studies of the subject, lists of control questions, practical skills and tests for self control and designed for improvement of self preparation to the course.Item Zoonotic and percutaneous infectious diseases : textbook for medical foreign student(2016) Kozko, Volodymyr; Katsapov, Dmytro; Bondarenko, Andriy; Gradil, Grigory; Yurko, Katerina; Mohylenets, Olena; Sokhan, Anton; Kopiychenko, YaroslavaThe material contained in the textbook reviews to the fundamental questions of zoonotic and percutaneous infectious diseases (etiology, epidemiology, pathogenesis, clinical manifestations, differential diagnosis and treatment). It would be helpful to medical students and interns.