03. Науково-дослідний інститут експериментальної та клінічної медицини ХНМУ

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Browsing 03. Науково-дослідний інститут експериментальної та клінічної медицини ХНМУ by Author "Myasoedov, Valeriy"

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    Changes in cell membranes of white blood cells treated with a common food additive E407a
    (2021) Tkachenko, Anton; Onishchenko, Anatolii; Posokhov, Yevgen; Roshal, Alexander; Myasoedov, Valeriy; Nakonechna, Oksana
    Objectives: To estimate the state of phospholipid bilayer of rats WBCs exposed to a common food additive E407a, which is used as a thickener and emulsifier, during 4 h using a fluorescent probe − ortho-hydroxy derivative of 2,5-diaryl-1,3-oxazole. Materials and methods: Steady-state fluorescence spectroscopy: a study by the environment-sensitive fluorescent probe − 2-(2′-hydroxy-phenyl)-5-phenyl-1,3-oxazole (probe O1O). Results: Changes are detected in the spectra of the fluorescent probe bound to rat WBCs treated with the solutions of E407a of various concentrations in comparison with the corresponding spectra of the probe incubated with the untreated leukocytes. The decrease in polarity and protondonor ability is observed in the lipid membranes of leukocytes in the region, where the probe locates. Conclusions: Our findings suggest a higher dehydration of leukocyte membranes of rats treated with a common food additive E407a at high concentrations and, thus, indicate that exposure to high doses of E407a leads to the increase in the lipid order (i.e. to decrease in fluidity) of the membranes of rat WBCs.
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    Cytotoxicity of Hybrid Noble Metal-Polymer Composites
    (2022-10-11) Tkachenko, Anton; Virych, Pavlo; Myasoedov, Valeriy; Prokopiuk, Volodymyr; Onishchenko, Anatolii; Butov, Dmytro; Kuziv, Yuliia; Yeshchenko, Oleg; Zhong, Sican; Nie, Guochao; Kutsevol, Nataliya
    The aim of the present research was to assess the cytotoxicity of gold and silver nanoparticles synthesized into dextran-graft-polyacrylamide (D-PAA) polymer nanocarrier, which were used as a basis for further preparation of multicomponent nanocomposites revealed high efficacy for antitumor therapy. The evaluation of the influence of Me-polymer systems on the viability and metabolic activity of fibroblasts and eryptosis elucidating the mechanisms of the proeryptotic effects has been done in the current research. The nanocomposites investigated in this study did not reduce the survival of fibroblasts even at the highest used concentration. Our findings suggest that hybrid Ag/D-PAA composite activated eryptosis via ROS- and Ca2+-mediated pathways at the low concentration, in contrast to other studied materials. Thus, the cytotoxicity of Ag/D-PAA composite against erythrocytes was more pronounced compared with D-PAA and hybrid Au/polymer composite. Eryptosis is a more sensitive tool for assessing the biocompatibility of nanomaterials compared with fibroblast viability assays.
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    Experimental Evaluation of Food-Grade Semi-Refined Carrageenan Toxicity
    (2021-10-16) Pogozhykh, Denys; Posokhov, Yevgen; Myasoedov, Valeriy; Gubina-Vakulyck, Galina; Chumachenko, Tetyana; Knigavko, Oleksandr; Polikarpova, Hanna; Kalashnyk-Vakulenko, Yuliia; Sharashydze, Ketino; Nakonechna, Oksana; Prokopyuk, Volodymyr; Onishchenko, Anatolii; Tkachenko, Anton
    Abstract: The safety of food additives E407 and E407a has raised concerns in the scientific community. Thus, this study aims to assess the local and systemic toxic effects of the common food additive E407a in rats orally exposed to it for two weeks. Complex evaluations of the effects of semi-refined carrageenan (E407a) on rats upon oral exposure were performed. Local effects of E407a on the intestine were analyzed using routine histological stains and CD68 immunostaining. Furthermore, circulating levels of inflammatory markers were assessed. A fluorescent probe O1O (2- (20-OH-phenyl)-5-phenyl- 1,3-oxazole) was used for evaluating the state of leukocyte cell membranes. Cell death modes of leukocytes were analyzed by flow cytometry using Annexin V and 7-aminoactinomycin D staining. Oral administration of the common food additive E407a was found to be associated with altered small and large intestinal morphology, infiltration of the lamina propria in the small intestine with macrophages (CD68+ cells), high systemic levels of inflammation markers, and changes in the lipid order of the phospholipid bilayer in the cell membranes of leukocytes, alongside the activation of their apoptosis. Our findings suggest that oral exposure to E407a through rats results in the development of intestinal inflammation.