Кафедра судової медицини, медичного правознавства імені заслуженого професора М.С. Бокаріуса
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Browsing Кафедра судової медицини, медичного правознавства імені заслуженого професора М.С. Бокаріуса by Author "Cherkashina, Lidiya"
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Item Cholinesterase in different types of the muscle tissue during the early postmortem period for diagnosis of death coming(Bangladesh Journal of Medical Science, 2021) Cherkashina, Lidiya; Shklyar, Anton; Sukhonosov, Roman; Miroshnikova, Olha; Naguta, Ludmyla; Olkhovskiy, Vasyl; Demikhova, Nadiia; Kuts, Larysa; Barchan, Ganna; Sukhomlyn, Ganna; Kyptenko, LudmilaThe purpose of the research: consisted in study of postmortem regularities in the content of cholinesterase in different types of muscle tissue (MT) for improving accuracy of determination of the prescription of death coming PDC.Materials and methods: The activity/level of cholinesterase was determined in homogenates of the myocardial (MMH), oesophageal (OMH), diaphragm (DMH) and intercostal muscles (IMH)within the early PMP (3-13 hours after the coming of death) on 30 human corpses. MTwas sampled in conditions of postmortem biopsy with use of special instruments; MT homogenates were prepared following the standard technique with subsequent determination of cholinesterase content in MT homogenates. Results and discussion: The analysis of postmortem changes in the content of cholinesterase in MT depending upon PDC revealed that after 3 hours from the moment of death coming its highest content was in muscles of the oesophagus, the least one being in MT of the intercostal muscles (respectively, (2,717.1±37.1) and (883.5±6.2) U/g, р<0.001). Levels of cholinesterase content in MT of the myocardium and diaphragm were rather close, though they differed (respectively, (1,213.8±8.8) and (1,512.8±11.5) U/g, р<0.05), and occupied an intermediate place between the corresponding values of MT of the intercostal muscles and oesophagus.A common pattern for the content of cholinesterase in different types of MT was characterized by a decrease of this content with an increase in PDC terms; besides, the dynamic lines of its changes, that we obtained, became basic ones for substantiating quantitative time dependencies and construction of relevant nomograms for forensic diagnosis of PDC by cholinesterase content in MT. Conclusions: It was proved that the content of cholinesterase in all MT homogenates, which we studied, changed regularly (and nonlinearly), but the initial and final levels of cholinesterase content differed depending upon the type of MT. Besides, the dynamics in changes of the content of cholinesterase within the time period of 3÷13 hours from the moment of death coming differed upon the type of MT too. Advantages of the technique consist in theintegrity of biochemical examination of different types of MT and simplicity in interpretation of findings. The application of the nomogram technique for assessing PDC by cholinesterase content in MT makes it possible to improve the accuracy of diagnosis for terms of the coming of death up to 60 minutes.Item The dynamics of changes in biochemical markers of the state of tissue in intercostal muscles during the early postmortem period(2020) Cherkashina, Lidiya; Konoval, Nataliia; Shklyar, Anton; Najar, Saleh; Haidash, Olena; Kuts, Larysa; Gortinskaya, Olena; Demikhova, NadiiaThe aim of study was to evaluate structural and biochemical changes in the tissue of intercostal muscles during the early postmortem period (PMP) – 3-13 hours. Material and methods. Absolute and relative values of the concentration of glycogen, acid phosphatase, lactate, lactate degydrogenase, lipofuscin and cholinesterase during the early PMP were determined on 30 human corpses by results of study of the tissue of intercostal muscles. Results. It was proved that the early PMP was characterized by proper biochemical and biophysical changes of the muscular tissue, the most demonstrative of them were as follows: a reduction in the concentration of glycogen and dynamic increases in the concentrations of lactate dehydrogenase and lipofuscin. For all six biochemical markers, representative absolute and relative values of their popstmortem content in homogenates of intercostal muscles depending upon the prescription of death coming were obtained. It was found out that the concentration of glycogen during the analysed time intervals ranged from (7.821±0.0649) mg/g in 3 hours after death coning to (3.204±0.030) mg/g in 13 hours after the coming of death, reliably (p<0.001) differing every 2 hours of PMP. The dynamics in the concentration of lactate were found to be demonstrative and characterized by its progressive (p<0.01) increase within the period of 9 hours from the moment of death coming: from (6.847±0.042) mmol/g after 3 hours to (12.960±0.085) mmol/g after 9 hours. The level of lipofuscin concentration in the analysed time intervals progressively rose too: from (2.258±0.031) U/g in 3 hours to (5.589±0.030) U/g in 13 hours, reliably (p<0.001) differing every 2 hours of PMP. Conclusions.Paired correlative indices between biochemical and biophysical markers of the state of tissue of intercostal muscles were examined in their systemic relationships and proper system-creating coefficients were determined by six time intervals of the early PMP, in its turn making it possible to substantiate those of them that were criterially significant for increasing the accuracy of diagnosis of prescription of death coming.Item The study of structural and biochemical changes in the muscular tissue of the oesophagus for solving the problem of diagnosing the prescription of death coming(2020) Cherkashina, Lidiya; Konoval, Nataliia; Shklyar, Anton; Kyrychenko, Igor; Haidash, Olena; Demikhova, NadiiaThe modern practice and tactics of forensic medical diagnosis require scientific substantiation of informative diagnostic criteria for assessing the prescription of death coming (PDC), first of all during the early postmortem period (PMP). The aim of the study was an evaluation of the concentration changes of biochemical markers (BCM) in muscular tissue (MT) of the oesophagus during the early postmortem period.