Investigation of Antiradiation and Anticancer Efficiency of Nanodiamonds on Rat Erythrocytes

Abstract

Given the existing insufficiency in radioprotective agents and the expansion of contacts between people interacting with ionizing radiation during treatment or in technological processes, the study of the radiobiological aspects of the application of nanodiamonds is actual. The study is aimed at detection of the anti-radiation effectiveness of ultradispersed nanodiamonds roduced by detonation synthesis during a general radiation damage of rats with a dose of 5.8 Gy. The study has been performed on the red blood cells of rats who received 1.0 ml of a suspension of nanodiamonds one time every 5 days prior to the irradiation. The suspension was diluted to C=0.01 % by distilled water or by saline to a final concentration of 0.9% NaCl. Blood sampling had been performed 30 days after irradiation. Investigation of changes in the dielectric properties and hydration of the red blood cells has been carried out by the method of microwave-dielectrometry at a frequency of 9.2 GHz. The results indicate that irradiation of rats results in an increase in the decrement of the static permittivity of the blood erythrocyte suspension by 37% with respect to those in the control group (intact rats). The degree of hydration of erythrocyte membranes in animals receiving nanodiamonds during the irradiation was found increased by 8.4%, relative to control, and decreased by 29% relative to irradiation without nanodiamons uptake. Based on the data on the dielectric permittivity and hydration changes in the patients with different types of cancer, the conclusion on radioprotective and anticancer properties of NDs has been derived.