Peculiarities of succinamide toxicokinetics as an antidiabetic agent upon single inhalaion exposure

Abstract

The toxicokinetics of such antidiabetic agents, as fi-phenylethylamide of 2-oxysuccinanilic acid ф -PhEA-OSA) and its metabolites (2-hydroxyphenylsuccinamide (2-HPhSA) andfi-phenylethylsuccinamide ф-PhESA)) upon a single inhalation influence of fi-PhEA-OSA on the male rodents at the levels Limac та Limch has been studied. The quantification of studied substances in the blood plasma of male rats was conducted with the use of high-performance liquid chromatography (HPLC) followed by the spectrophotometric determination. The compounds were identified in blood plasma within 30 mins after the inhalation; the maximum plasma concentration of fi-PhESA was estimated to be higher than the concentration of fi-PhEA-OSAA and 2-HhHS. The compounds were shown to circulate for a long period in the blood stream; the 2-HPhS was counted to have the longest half-life in average; the biotransformation of в -PhEA-OSAA was calculated to be more intensive in comparison with its excretion.