TLR expression on peripheral blood monocytes in patients with psoriasis

Abstract

Toll-like receptors (TLRs) are signalling pattern-recognition receptors, which play an important part in initiating the immune response in psoriasis. The available literature has little information about study of these receptors in blood. The purpose of the present work was to study the level of expression of TLR2 and TLR4 types on blood monocytes in a psoriasis patient. Within 2016-2018, TLR2 and TLR4 were examined thrice in the blood of a patient with psoriasis during its exacerbation before the beginning of his treatment. The expression of surface receptors CD282 (TLR2) and CD284 (TLR4) on peripheral blood monocytes was studied by the method of flow cytometry with use of monoclonal antibodies. The obtained data show that the expression of TLR2 on peripheral blood monocytes was high, while that of TLR4 was mostly within its reference values of 3.3±0.1 mfi. Hence, the conducted studies have shown that the expression of TLR2 on peripheral blood monocytes in all three studies was high, thereby demonstrating involvement of this factor into the pathogenesis of the above disease. During all three blood examinations, monocytes revealed an extremely high intensity of TLR2 fluorescence on studied cells that exceeded the same intensity in healthy subjects by 1.5-2 times. The expression of TLR4 on peripheral blood monocytes during all three studies was within its reference values, this fact demonstrating absence of any information value of the above factor in the pathogenesis of psoriasis.