Please use this identifier to cite or link to this item:
http://repo.knmu.edu.ua/handle/123456789/22345
Title: | HSP90α overexpression in small intestinal mucosa and high blood serum levels of HSP70 and 8-isoprostane in carrageenan-induced intestinal inflammation |
Authors: | Tkachenko, Anton Onishchenko, Anatolii Gorbach, Tetiana Nakonechna, Oksana Shekhovtsova, E. Gubina-Vakulyck, Galina |
Keywords: | food additive heat shock proteins 8-isoprostane intestinal inflammation oxidative stress |
Issue Date: | 2019 |
Publisher: | Bioflux |
Citation: | HSP90α overexpression in small intestinal mucosa and high blood serum levels of HSP70 and 8-isoprostane in carrageenan-induced intestinal inflammation / A. S. Tkachenko, A. I. Onishchenko, T. V. Gorbach, O. A. Nakonechna, E. V. Shekhovtsova, G. I. Gubina-Vakulyсk // HVM Bioflux. – 2019. – Vol. 111, issue 1. – P. 1–5. |
Abstract: | Aims. The aim of our research was to evaluate HSP90α expression in small intestinal tissue, levels of HSP70 and 8-isoprostane in blood serum of rats with chronic carrageenan-induced enteritis. Methods. We determined immunohistochemically HSP90α expression in small intestine of ten rats with intestinal inflammation caused by the four-month-long oral administration of k-carrageenan-containing 1% processed Eucheuma seaweed solution and compared it with the small intestinal HSP90α expression in ten healthy animals. Levels of 8-isoprostane and HSP70 were determined in blood serum of animals from both groups using commercially available ELISA kits. Results. HSP90α overexpression was observed in small intestine of rats with carrageenan-induced enteritis. The disease was also associated with the elevated circulating levels of 8-isoprostane, indicating the development of oxidative stress. The level of HSP70 in blood serum was also found to be higher in animals with carrageenan-induced intestinal inflammation compared with control rats. Conclusion. Our findings suggest that HSP90α and HSP70 play an important role in the pathogenesis of chronic carrageenan-induced enteritis. |
URI: | https://repo.knmu.edu.ua/handle/123456789/22345 |
Appears in Collections: | Наукові праці. Кафедра біологічної хімії |
Files in This Item:
File | Description | Size | Format | |
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Ткаченко.pdf | 1,12 MB | Adobe PDF | View/Open |
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