Investigation of fatty acid composition of herb and roots of fetid meadow rue (thalictrum foetidum l.)

Abstract

The manuscript presents the results of the determination of fatty acids in the herb and roots of fetid meadow rue. Investigation has been performed by the gas chromatography-mass spectrometry (GC/MS) that is based on the getting of fatty acid methyl esters and their further analysis. Identification of fatty acid methyl esters was carried out by the comparison of the retention times of standard mixture of fatty acid methyl esters of bacteria (Supelco, USA) and by the usage of mass spectral library NIST 02. From the results of the chromatographic analysis 34 lipophilic compounds were identified in the herb of fetid meadow rue and 44 lipophilic compounds were identified in the roots of this plant. It was found that Hexadecanoic acid, methyl ester (palmitic acid, methyl ester) and 9,12,15-Octadecatrienoic acid, methyl ester (linolenic acid, methyl ester) are abundant in the herb of fetid meadow rue in comparison with other identified fatty acids. Concentrations of these acids are 3.582 mg/g and 3.830 mg/g, respectively. During the analysis of fatty acid composition of the meadow rue it was established that its composition is more varied although identified compounds are contained in much less quantities. Among the identified fatty acids 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (linolenic acid, methyl ester) (1.066 mg/g), 9-Octadecenoic acid (Z)- methyl ester (oleic acid, methyl ester) (0.842 мг/г), Hexadecanoic acid, methyl ester (palmitic acid, methyl ester) (0.801 mg/g) are found in higher quantity